A fundamental question in protein evolution is whether nature has exhaustively sampled nearly all possible protein folds throughout evolution, or whether a large fraction of the possible folds remains unexplored. To address this question, we defined a se
single chain precursors. In this work, we developed a unique, efficient, and convenient interchain disulfide pairing strategy to direct peptide heterodimerizations. By combining the disulfide pairing chemistry with de novo protein design, we can exploit the dimeric structure space for designing peptide ...
Genomics. Next-gen Sequencing. Howard Salis, Ph.D. Chemical Engineer. Synthetic Biologist. Software developer. Entrepreneur. Professor. Published Applications Contact Us 200 Innovation Blvd. Suite 239. State College, PA. 16803 salis@denovodna.com ...
Advances in the so-called ‘de novo’ design problem have recently been brought forward by developments in artificial intelligence. Generative architectures, such as language models and diffusion processes, seem adept at generating novel, yet realistic proteins that display desirable properties and ...
De novo design of an intercellular signaling toolbox for multi-channel cell–cell communication and biological computationArticle Open access Published: 24 August 2020 Volume 11, article number 4226, (2020) Cite this article Download PDF You have full access to this open access article ...
The amino-acid sequence of a protein encodes information on its three-dimensional structure and specific functionality. De novo design has emerged as a method to manipulate the primary structure for the development of artificial proteins and peptides with desired functionality. This paper describes the...
methods and caveats for thede novosynthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scalede novoDNA constructs, as well as the challenges and opportunities that lie ...