A fundamental question in protein evolution is whether nature has exhaustively sampled nearly all possible protein folds throughout evolution, or whether a large fraction of the possible folds remains unexplored. To address this question, we defined a se
Design of pseudosymmetric protein hetero-oligomers Article Open access 18 December 2024 De novo design of obligate ABC-type heterotrimeric proteins Article Open access 15 December 2022 Accurate de novo design of heterochiral protein–protein interactions Article Open access 14 August 2024 Introducti...
Researchers from the University of Colorado Boulder utilized theOperon Calculatorand theRBS Library Calculatorto design a synthetic operon that expresses 7 urease enzymes responsible for CaCO3 production in E. coli. By varying enzyme expression levels, they found an inverse relationship between urease ex...
De novo design of an intercellular signaling toolbox for multi-channel cell–cell communication and biological computationArticle Open access Published: 24 August 2020 Volume 11, article number 4226, (2020) Cite this article Download PDF You have full access to this open access article ...
The amino-acid sequence of a protein encodes information on its three-dimensional structure and specific functionality. De novo design has emerged as a method to manipulate the primary structure for the development of artificial proteins and peptides with desired functionality. This paper describes the...
Efforts to construct synthetic biological circuits with more complex functions have often been hindered by the idiosyncratic behavior, limited dynamic range and crosstalk of commonly utilized parts. Here, we employ de novo RNA design to develop two high-performance translational repressors with sensing ...
methods and caveats for thede novosynthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scalede novoDNA constructs, as well as the challenges and opportunities that lie ...