Whole body purine synthesis de novo is accelerated in both these groups of patients. A strain of mice with an experimentally produced mutation at the HPRT locus showed some residual 'apparent HPRT activity' in brain, liver, testicular, splenic, kidney and ovarian tissues but not in erythrocyte ...
Allsop J. & Watts R.W. (1986) Purine de novo synthesis and sal- vage during testicular development in the rat. Advances in Experimental Medicine and Biology 195, 325-28.Purine de novo synthesis and salvage during testicular development in the rat. Allsop J,Watts R W. Advances in ...
Natsumeda, Y., Ikegami, T., and Weber, G. Purine synthetic capacities of de novo and salvage pathways in rat hepatoma 3924A cells. Adv. Exp. Med. Biol., 195B: 371-376, 1986.Natsumeda, Y., Ikegami, T., and Weber, G. Purine synthetic capacities of de novo and salvage pathways ...
Thus, a combination approach to block both the de novo and salvage pathways of pyrimidine ribonucleotide synthesis could be a reasonable strategy to consider for cancer therapy. Indeed, such a combination has been proven effective in cell culture and in vivo cancer models [[17], [18], [19],...
There are two major pathways to synthesize nucleotides in cells: the de novo nucleotide biosynthetic pathway and the salvage synthesis pathway.1, 2, 3 The salvage synthesis pathway uses exogenous nucleic acid raw materials or endogenous nucleic acid degradation intermediates as substrates. Most ...
Factors controlling relative flux rates of the de novo and salvage pathways of purine nucleotide biosynthesis during animal cell growth are not fully understood. To examine the relative role of each pathway for cell growth, three cell lines including CHO K1 (a wild-type Chinese hamster ovary fibro...
Procedures for assaying the rate of purine de novo synthesis in cultured fibroblast cells have been compared. These were (i) the incorporation of [ 14C]glycine or [ 14C]formate in α-N- formylglycinamide ribonucleotide (an intermediate in the purine synthetic pathway) and (ii) the incorporation...
The key difference between de novo and salvage pathway is that de novo synthesis of purine nucleotides refers to the process that utilizes small molecules
(2005) Progres- sion of programmed cell death in tobacco BY-2 cells is delin- eated by specific changes in de novo and salvage synthesis of purine nucleotides. Physiologia Plantarum 123, 254-261.Claudio Stasolla,Natalia Loukanina,Edward C. Yeung and Trevor A. Thorpe.Progression of programmed ...
These results suggest that the inhibition of de novo purine synthesis by MTA is due to cleavage of the MTA to adenine by the MTAP enzyme. The adenine so formed can be converted to 5'-AMP in a PRPP-dependent reaction catalysed by the purine salvage enzyme APRT.Inhibition of growth of the...