（de novo 的意思是全新，assembly是序列拼接），即在没有参考序列的情况下进行序列拼接，对未知基因组...
生物测序中的de novo genome assembly，简单来说，就是对未知基因组进行从无到有的序列构建过程。"de novo"意味着在没有预先存在的参考序列的情况下，通过生物信息学技术，将测序片段拼接起来，形成完整的基因组图谱。如果已经有人类或其他物种的基因组作为参考，对它们的组装则不属于de novo，而是ab ini...
de novo assembly是新的基因组装配，（de novo 的意思是全新，assembly是序列拼接），即在没有参考序列的情况下进行序列拼接，对未知基因组序列进行测序，利用生物信息学分析手段，对序列进行拼接、组装，从而获得其基因组的图谱。但这是个相对的概念，比如如果有了人类基因组作参考，那拼chimpanzee的就不...
De novo genome assembly 在生物测序中的意思是全新的基因组组装。这意味着从原始的测序数据中重新构建基因组序列，而不是依赖于已有的参考基因组序列。下面详细介绍这一概念。一、基本定义 De novo genome assembly 是一种生物信息学技术，它通过对原始测序数据进行分析和组装，生成全新的基因组序列。在这...
de novo genome assembly指的是从头测序，是在没有参考序列的情况下对序列进行拼接。比如说，对未知的基因组进行测序，再通过生物信息学的方法进行拼接。coverage是覆盖度，由于测序长度，拼接之间的gap，重复序列等的问题，有部分基因我们没办法得到，这就有了覆盖度。比如说，测某一物种得到的覆盖度是98...
As more and more genomes are sequenced, reference-guided assembly approaches can be used to assist the assembly process. However, previous methods mostly focused on the assembly of other genotypes within the same species. We adapted and extended a reference-guided de novo assembly approach, which ...
参考基因组:Reference genome 人类参考基因组是来自多个匿名个体的复合基因组 image.png 那么是来自每个物种的单个或多个参考基因组吗? 当前参考基因组B73不代表玉米中的所有单倍型。 现在有>26个玉米基因组组装体。 个体基因组可以表示为单倍型等位基因的路径。A individual genome can be represented as a path of...
Possibility to generate large RNA-seq datasets has led to development of various reference-based and de novo transcriptome assemblers with their own strengths and limitations. While reference-based tools are widely used in various transcriptomic studies, their application is limited to the model organis...
We introduce a generative probabilistic model of read generation from environmental samples and present Genovo, a novel de novo sequence assembler that discovers likely sequence reconstructions under the model. A Chinese restaurant process prior accounts for the unknown number of genomes in the sample. ...
In conclusion, NOVOPlasty is the sole de novo assembler that provides a fast and straightforward extraction of the extranuclear genomes from WGS data in one circular high quality contig. The software is open source and can be downloaded at https://github.com/ndierckx/NOVOPlasty. 展开 ...