we used mass photometry (MP). We measured the assembly state of five of the rings using MP13in the presence and absence of tenfold molar excess of effector. There was a clear shift in oligomeric state in all cases, with the predominant mass...
(ITC), yielding a measured dissociation constant ofKD= 0.8 ± 0.1 μM, and a molar ratio of one ligand per protein molecule (Fig.5b). ThisKDvalue of compound1confirmed the observed direct receptor modulation in the functional reporter gene assay. Compound2demonstrated a noteworthy...
The cytopathic effects induced by TcdB1 or TcdB4 at a concentration of 100 pM were quantified in HeLa CSPG4‒/‒ cells using the cell-rounding assay. Various concentrations of 43829, H1, and H3 were included, and the molar ratio of minibinder to TcdB was plotted on the X axis. ...
1 mM Sodium Palmitate/0.17 mM BSA solution (6:1 molar ratio Palmitate:BSA) was prepared using ultra-fatty acid-free BSA (SIGMA ALDRICH, A6003-25G). Briefly, BSA was dissolved in 150 mM NaCl solution. Half of the solution was conjugated with Sodium Palmitate (SIGMA ALDRICH, P-97...
PROTAC X1 exhibits sustained low-nanomolar binding affinity and exceptional selectivity toward PTPN1 and PTPN2 in in vitro settings. Furthermore, it has demonstrated a nano-molar half-maximal degradation concentration (DC50) in Jurkat T cells and numerous cancer cell lines, while concurrently ...
The observed enthalpy was plotted as a function of the molar ratio of PAMI to PPA, yielding the ligand to enzyme binding stoichiometry of 1.08. The binding strength of PAMI calculated from the plot corresponds to the association constant KA = 1.65 × 105 M−1 (Kd of 6.1 μM). The ...
To obtain biotinylated-VCB, protein was mixed with EZ-link NHS-PEG4-biotin (Thermo Scientific) in a 1:1 molar ratio and incubated at room temperature for 1 h. The reaction was quenched using 1 M Tris-HCl, pH 7.5, and unreacted NHS-biotin was removed with a PD-10 MiniTrap desalting...
The term “KD”, as used herein, is intended to refer to the dissociation constant, which is obtained from the ratio of Kd to Ka (i.e., Kd/Ka) and is expressed as a molar concentration (M). KD values for antibodies can be determined using methods well established in the art. A me...
“μM” means micromolar, “nM” means nanomolar, “M” means molar, “mmol” means millimole(s), “μmol” mean micromole(s), “ng” means nanogram, “mg” means milligram(s), “g” means gram(s), “kB” means kilobase(s), “mU” means milliunit(s), and “U” means unit(...
4‐NBA could bind PnbX at a 1:1 molar ratio with K D = 26.7 ± 4.2 nM. Low‐nanomolar levels of 4‐NBA induced the transcription of the pnb operon in strain LMS‐CY. PnbX bound a palindromic sequence motif (5''‐TTACGTTACA‐N 8 ‐TGTAACGTAA‐3′) that encompasses the pnb ...