we used mass photometry (MP). We measured the assembly state of five of the rings using MP13in the presence and absence of tenfold molar excess of effector. There was a clear shift in oligomeric state in all cases, with the predominant mass...
which were in the nanomolar range (Supplementary Fig.8). The best candidate was cyclic peptide11, with aKiof 82 ± 10 nM for human α-thrombin. Group 1 contained the most active peptides (cyclic peptides11–16), which shared the amino acid motif Cys-Phe (or Cys-Pal)...
Specific binding of FITC-ubiquitin to CXCR4 was measured in the presence of a 30-fold molar excess of unlabeled ubiquitin (Sigma). Samples were incubated 30 min at 4 °C and washed twice with 500 μl cold PBS. Fluorescence intensities of 1 × 104 cells were determined using flow cytometry...
The smaller the dissociation constant, the more tightly bound the peptide is; for example, a peptide with a nanomolar (nM) dissociation constant binds more tightly than a peptide with a micromolar (μM) dissociation constant. In one embodiment, the present PMMA-binding peptides have a KD ...
(C-Ab). The C-Ab should be in molar excess over the highest concentration of analyte expected to be found in a clinical sample. The mixture is then incubated during which time a fraction of the C-Ab will immunochemically bind to analyte while the other fraction of C-Ab will remain ...
The classical protein extraction protocol using mechanical homogenisation, sonication and high molar urea releases a variety of stress proteins, notably of the Hsp70-family, which originate from different cellular compartments, such as the cytosol, nucleus, mitochondria or endoplasmatic reticulum (ER). ...
Sensitivity and molar extinction coefficient are 0.0055 渭g As/ml and ... PK Gupta,PK Gupta - 《Microchemical Journal》 被引量: 23发表: 1986年 Spectrophotometric determination of ziram (dithiocarbamate fungicide) by thiocyanate and rhodamine 6G method A sensitive spectrophotometric method has been ...
On the background of recently described relevant intracellular FTY720 effects in the nanomolar range and the prolonged application in multiple sclerosis, this data showing a substantial intracellular accumulation of FTY720, has to be considered for benefit/risk ratio estimates....
(ITC), yielding a measured dissociation constant ofKD= 0.8 ± 0.1 μM, and a molar ratio of one ligand per protein molecule (Fig.5b). ThisKDvalue of compound1confirmed the observed direct receptor modulation in the functional reporter gene assay. Compound2demonstrated a noteworthy...
(right) of tenfold molar excess of peptide.f, Characterization of sr508, with data following column organization ofa–e, from left to right: design model in the unbound X2 state, design model in the bound Y2P4 state, nsEM 2D class averages for sr508 in the absence (left) and ...