品系全称 FVB-Tg(Ddx4-cre)1Dcas/J 鼠来宝品系编号 YDS0228 品系来源 Cyagen方案,但未搜到,jax有相关信息,Jax. 006954 品系背景 C57BL/6 常用名 Vasa-Cre NCBI ID 未知 基因名 DEAD box helicase 4 基因位置 18号染色体 基因表达 cre,cre recombinase,bacteriophage P1 表达部位 雌性和雄性生殖细胞从胚胎E15...
Ddx4基因在果蝇、斑马鱼、小鼠等生物的生殖细胞中特异性表达。在小鼠中,Ddx4在原始生殖细胞(PGCs)中表达,我们可以在小鼠12.5d胚龄时就可以在生殖脊中检测到Ddx4的表达,整个表达一直维持至后期精子细胞和原代卵母细胞。 Ddx4-Cre(又可称为Vasa-Cre)转基因小鼠在小鼠Ddx4启动子的调控下,在雄性和雌性胚胎生殖细胞中...
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将P2A-Cre-T2A-tdTomato插入到小鼠Ddx4基因终止密码子处。 应用领域:Cre工具鼠,生殖系统 *使用本品系发表的文献需注明: Ddx4-P2A-Cre-2A-tdTomato mice (Cat. NO. NM-KI-225028) were purchased from Shanghai Model Organisms Center, Inc..
Deregulation of transposable elements in Ddx4-Cre;Dcr1fx/fx (−/−) mutant spermatocytes.Yannick, RomeroOliver, MeikarMarilena, D. PapaioannouBéatrice, ConneCorinne, GreyManuela, WeierFrançois, PralongBernard, de MassyHenrik, Kaessmann
Meiotic progression defects in Ddx4-Cre;Dcr1fx/fx (−/−) mutant testes.Yannick, RomeroOliver, MeikarMarilena, D. PapaioannouBéatrice, ConneCorinne, GreyManuela, WeierFrançois, PralongBernard, de MassyHenrik, KaessmannJeanDominique, Vassalli...
Generation and characterization of a Ddx4‐iCre transgenic line for deletion in the germline beginning at genital ridge colonizationdoi:10.1002/dvg.23511Burnet, GuillaumeFeng, Chun‐Wei AllenCheung, Ka Man FionaBowles, JosephineSpiller, Cassy M....
We found the Ddx4iCreJoBo allele to be hypomorphic for Ddx4 expression and homozygous males, but not females, were infertile. Using two reporter lines (R26RLacZ and R26RtdTomato) and a floxed gene of interest (Criptoflox) we found ectopic activity in multiple organs; global recombination (...
Ddx4 was specifically expressed in both female and male germ cell lineages. We mated the CreERT2 mice with R26GRR mice, expressing enhanced green fluorescent protein (EGFP) and tDsRed before and after Cre recombination. We found tDsRed signals in the testes and ovaries of tamoxifen‐treated ...
To obtain full understanding of the molecular mechanisms underlying germ cell development, the Cre/loxP system has been widely applied for conditional knock-out mouse studies. In this study, we established a novel knock-in mouse line, B6-Ddx4( em1(CreERT2)Utr), which expresses CreERT2 ...