在小鼠中,Ddx4在原始生殖细胞(PGCs)中表达,我们可以在小鼠12.5d胚龄时就可以在生殖脊中检测到Ddx4的表达,整个表达一直维持至后期精子细胞和原代卵母细胞。 Ddx4-Cre(又可称为Vasa-Cre)转基因小鼠在小鼠Ddx4启动子的调控下,在雄性和雌性胚胎生殖细胞中特异表达Cre重组酶。Ddx4-Cre小鼠可用于在雄性和雌性生殖细胞...
品系全称 FVB-Tg(Ddx4-cre)1Dcas/J 鼠来宝品系编号 YDS0228 品系来源 Cyagen方案,但未搜到,jax有相关信息,Jax. 006954 品系背景 C57BL/6 常用名 Vasa-Cre NCBI ID 未知 基因名 DEAD box helicase 4 基因位置 18号染色体 基因表达 cre,cre recombinase,bacteriophage P1 表达部位 雌性和雄性生殖细胞从胚胎E15...
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However, a recent study using Ddx4-Cre;Rosa26 reporter mice concluded that such germ cells do not exist. Given the disparity in conclusions drawn in this study compared with others, we felt it was important to re-assess the utility of Ddx4-Cre;Rosa26 reporter mice for identification of ...
Ddx4-P2A-Cre-2A-tdTomato 品系名:C57BL/6Smoc-Ddx4em(P2A-Cre-T2A-tdTomato)1Smoc 品系状态:活体 | 目录号:NM-KI-225028 Ddx41-Flox 品系名:C57BL/6Smoc-Ddx41em2(flox)Smoc 品系状态:活体 | 目录号:NM-CKO-241380 Ddx41-KO 品系名:C57BL/6Smoc-Ddx41em1Smoc ...
Ddx4-P2A-Cre-2A-tdTomato 品系名:C57BL/6Smoc-Ddx4em(P2A-Cre-T2A-tdTomato)1Smoc 品系状态:活体 | 目录号:NM-KI-225028 Ddx41-Flox 品系名:C57BL/6Smoc-Ddx41em2(flox)Smoc 品系状态:活体 | 目录号:NM-CKO-241380 Ddx41-KO 品系名:C57BL/6Smoc-Ddx41em1Smoc ...
Deregulation of transposable elements in Ddx4-Cre;Dcr1fx/fx (−/−) mutant spermatocytes.Yannick, RomeroOliver, MeikarMarilena, D. PapaioannouBéatrice, ConneCorinne, GreyManuela, WeierFrançois, PralongBernard, de MassyHenrik, Kaessmann
Meiotic progression defects in Ddx4-Cre;Dcr1fx/fx (−/−) mutant testes.Yannick, RomeroOliver, MeikarMarilena, D. PapaioannouBéatrice, ConneCorinne, GreyManuela, WeierFrançois, PralongBernard, de MassyHenrik, KaessmannJeanDominique, Vassalli...
We found the Ddx4iCreJoBo allele to be hypomorphic for Ddx4 expression and homozygous males, but not females, were infertile. Using two reporter lines (R26RLacZ and R26RtdTomato) and a floxed gene of interest (Criptoflox) we found ectopic activity in multiple organs; global recombination (...
To obtain full understanding of the molecular mechanisms underlying germ cell development, the Cre/loxP system has been widely applied for conditional knock-out mouse studies. In this study, we established a novel knock-in mouse line, B6-Ddx4( em1(CreERT2)Utr), which expresses CreERT2 ...