Our intuitive interface enables seamless navigation through symptoms and systems, Lab tests accompanied by detailed explanations, algorithms, and key diagnostic criteria to enhance your clinical acumen. Engage in interactive case studies to apply your knowledge and receive personalized feedback. Test yoursel...
Our intuitive interface enables seamless navigation through symptoms and systems, Lab tests accompanied by detailed explanations, algorithms, and key diagnostic criteria to enhance your clinical acumen. Engage in interactive case studies to apply your knowledge and receive personalized feedback. Test yoursel...
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Fig. 1: DDX18 is required for hESC maintenance. aWestern blot analysis of pluripotency and lineage-specific transcription factors in control (shLuc) and DDX18 knockdown (KD) cells with two independent shRNAs (shDDX18#1 and shDDX18#3). β-Actin was used as the loading control. The experime...
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Pluripotent stem cells possess a unique nuclear architecture characterized by a larger nucleus and more open chromatin, which underpins their ability to self-renew and differentiate. Here, we show that the nucleolus-specific RNA helicase DDX18 is essential for maintaining the pluripotency of human embr...
Given the low dynamics of R475G hollow condensates, we felt this would also likely be the case for these condensates. Thus, TRIM32 (an E3 ubiquitin ligase) and ANKRD52 (a regulatory protein of protein phosphatase 6), two top targets enriched in APEX2-R475G mass spec pulldown (Fig. 5b)...
(infected cells) antibody controls. DDX3X depletion in the cell and virions was confirmed by probing DDX3X in the same samples.bImage Lab version 5.0 was then used to quantify the Western blots (note that detection of the proteins was done on an instrument with a 4-log linear range, not...
These findings were also verified with FUNCAT assays, which detected some cells with AHA signal at granules (Figure S6G, arrowheads), as well as examples, even within the same cell, where this was not the case. These data suggest that DDX3X RNP granules are heterogeneous, and unlike ...
Our intuitive interface enables seamless navigation through symptoms and systems, Lab tests accompanied by detailed explanations, algorithms, and key diagnostic criteria to enhance your clinical acumen. Engage in interactive case studies to apply your knowledge and receive personalized feedback. Test yoursel...