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To directly study the molecular exchange between the cytoplasm and the hollow condensates formed by DDX3X mutants, we performed “full bleach” fluorescence recovery after photobleaching (FRAP) experiments, in which we bleached an entire hollow condensate and measured the dynamics of fluorescence signal...
To substantiate our model whereby DDX18 depletion could cause the NAD and LAD exchange andHOXAgene derepression (Fig.6c), we performed the following studies. First, we performed additional Hi-C data analysis on chromatin interactions involving theHOXAlocus and revealed that, despite the lack of a...
An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs EMBO J., 29 (2010), pp. 1713-1725 CrossrefView in ScopusGoogle Scholar Chen et al., 2016 Q. Chen, L. Sun, Z.J. Chen Regulation and function of the cGAS-STING pathway of cytosolic DNA...
The 6xHis-MBP tag was cleaved overnight by tev protease at a 1:40 tev:protein ratio (w/w), and then dialyzed into ion exchange low salt buffer (200 mM NaCl, 20 mM HEPES ph7, 10% Glycerol, 0.5 mM TCEP). The sample was loaded onto a GE HiTrap heparin column (GE, 17-0406-01)...
The Morningstar Medalist Rating for funds is the summary expression of Morningstar’s forward-looking analysis of investment strategies as offered via the specific vehicles, which can include but are not limited to open-end funds, closed-end funds, exchange-traded funds, and separately managed acco...
After sonication, cell lysates were centrifuged through a 3 kDa filter (Millipore, UFC900324) to remove excess biotin and to exchange the buffer to 50 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.2% SDS, containing 1X Protease Inhibitor Cocktail. Cell lysates were incubated with MagReSyn Streptavidin...