RT-PCR users can select a single assay ID for specific primer/probe set against a single variant of interest, or a specific group of variant-associated mutations. RT-PCR assays were wet-lab verified with theReliance One-Step Multiplex Supermixand Bio-Rad’s Exact Diagnostics variant assay cont...
Template sequence heterogeneity also often limits the design of qRT-PCR assays, which requires placement of primer and probe sequences in highly conserved regions. Third, in some diseases, tis- sue sites have been found to serve as better predictors of disease outcome and...
For the qPCR mutation detection, the Quantimix easy probes kit (Biotools, Madrid, Spain)with the Thermo Fisher ScientificBRAFp.V600E probe was used. The reactions were carried out in a CFX Connect Real-Time PCR Detection System (Bio-Rad, Madrid, Spain). The normalized expression and raw fl...
The JCPyV real-time PCR assay performed at the NIH Clinical Center Microbiology Service targets a 78 base pair region of the JCPyV large T antigen gene [9] using the ABI 7500 Real-Time PCR System. This PCR system utilizes a single probe specific for the JCPyV sequence between the forward...