ddPCR Expert Design Assays are designed specifically for Droplet Digital PCR by the experts that developed the technology. PrimePCR Assays and Panels Experimentally validated real-time PCR PCR primer and probe assays for mutation detection, gene expression, copy number variation. Design your own custom...
Presumably, ddPCR could not well detect the L858R allele in this case because the mutant allele- specific MGB probe specifically bound to the c.2573T > G targeting sequence but could not efficiently bind to the two-base substitution-positive (c.2573_2574delinsGA) target DNA sequence (Fig...
In addition, the HEX probe assay for the human Ribonuclease P protein subunit p30 (RPP30) (ddPCR CNV Assay Validated, BioRad, Hercules, CA, USA) was used as the human housekeeping gene. The duplex PCR was performed using the One-step RT-ddPCR Advanced Kit for Probes (BioRad, Hercules,...