RT-PCR users can select a single assay ID for specific primer/probe set against a single variant of interest, or a specific group of variant-associated mutations. RT-PCR assays were wet-lab verified with theReliance One-Step Multiplex Supermixand Bio-Rad’s Exact Diagnostics variant assay cont...
The proposed new diagnostic tool based on the CRISPR-Cas13a complex was compared with qPCR sensitivity and specificity. This technique is widely used as a diagnostic method to detect the WT and mutant alleles separately using different probes. The technique is able to detect until at least a VAF...
4.1. Design of Primers and Probes: Aspergillus Species-specific assays were designed employing the NCBI primer-blast software using the 18S ITS sequence of Aspergillus fumigatus (accession number NR_121481) and Aspergillus terreus (accession number NR_131276). Primers and probes annealing to sequence...
DNA methylation profiling as a tool for testicular germ cell tumors subtyping. Epigenomics 2018, 10, 1511–1523. [Google Scholar] [CrossRef] Ellinger, J.; Albers, P.; Perabo, F.G.; Muller, S.C.; von Ruecker, A.; Bastian, P.J. CpG island hypermethylation of cell-free circulating ...