The package is designed for the data analysis of quantitative real-time PCR (qRT-PCR) experiemtns in Bioconductor. With the ddCt package, one can acquire the relative expression of the target gene in different samples. This vignette mainly dicusses the principles of the ddCt algorithm and ...
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Both absolute and relative quantication have been used to analyse the data from the quantitative real{time PCR (qRT{PCR, or RT{PCR for short) experiments. The 2 CT algorithm, also known as the the delta-delta-Ct or ddCt algorithm, is a convenient method to analyze the relative changes ...
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method Quantitative real time PCR (qRT-PCR) was performed to analyse the expression of HRI, GADD34, CHOP, and ATF4 and relative expression was calculated by 2-DDCt method. We also deve...