应用核蛋白提取试剂盒提取细胞蛋白,进行ELISA实验。首先用浓度100 μL/孔的抗DcR3 单克隆抗体包被ELISA板,置于37 ℃下4 h;再加入5%小牛血清置于37 ℃下封闭40 min,封闭时将封闭液加满各反应孔,封闭结束后用洗涤液满孔洗涤3遍,每遍3 ...
Moreover, recombinant DcR3.Fc and heparan sulfate proteoglycan binding domain of DcR3.Fc (HBD.Fc) fusion proteins attenuated weight loss and protected mice from IAV-induced lethality. Thus, DcR3-mediated protection is not only via suppression of proinflammatory cytokine and chemokine release, but ...
Cells were washed again and fluorescence signal of the stained cells was detected using a BD Bio- sciences FACSCalibur. Initial experiments showed no dif- ference between cells stained with rhIgG-Fc + anti-human Fc FITC and anti-Fc FITC alone, so anti-Fc alone was used as the...
PKC-delta is activated via cross-linking of heparan sulfate proteoglycan (HSPG) on DCs, because recombinant protein containing the heparin-binding domain (HBD) of DcR3 and the Fc portion of IgG(1), the HBD.Fc fusion protein, is also able to trigger DC apoptosis. This provides the first ...