②加入少量 Trypsin-EDTA solution,略盖过细胞即可,室温放置 0.5~2min, 不同的细胞消化时间有所不同。 ③显微镜下观察,细胞明显收缩,并且肉眼观察培养器皿底部发现细胞的形态发生明显的变 化;或者用枪吹打细胞发现细胞刚好可以被吹打下来,吸除胰酶细胞消化液。加入含血清的完全细胞培养液,吹打下细胞,即可直接用于后续...
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution H2DCFDA (DCFH-DA)注意事项及储存 1.请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C...
Shipping ConditionEvaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request 西默科技核心团队在试剂领域工作多年,行业经验丰富,富于创新。西默科技是一家开放的试剂平台,与全球优秀的科学家和工程师合作,为中国提供zhuo越的实验室产品和服务。 特种试剂...
After light irradiation, the fluorescence signal of DCFH-DA solution increased significantly with pH value higher than 7.4. The increase of fluorescence signal was attributed to a self-oxidization process of DCFH-DA, which transferred dichlorodihydrofluorescein (DCFH) to 2鈥 ,7鈥 -dichlorofluorescein...
活性氧(ROS)检测试剂盒-DCFHDA的相关产品:斐林试剂(Fehling's solution)细胞凋亡阳性对照试剂盒Annexin V-EGFP细胞凋亡检测试剂盒Caspase 8 活性检测试剂盒活细胞染色液(Hoechst 33342)(100×)Annexin V-APC/7-AAD双染细胞凋亡检测试剂盒Caspase 8分光光度法检测试剂盒细胞凋亡线粒体膜电位检测试剂盒(JC-1)FI 活...
H2DCFDA (DCFH-DA) 属于绿色荧光染料,是一种检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm),具有细胞膜渗透性。 规格价格库存数量 50 mg ¥ 415 现货 100 mg ¥ 583 现货 1 mL x 10 mM (in DMSO) ¥ 456 现货 大包装 & 定制 ...
3. 去除培养基,用HBSS清洗细胞2次,添加Photo-oxidation Resistant DCFH-DA Dye Working solution。 4. 37℃、5% CO2培养箱内培养30 min。 5. 去除培养基,用HBSS清洗细胞2次,添加Highly Sensitive DCFH-DA Dye Working solution。 6. 37℃、5% CO2培养箱内培养30 min。
30% H2O2Solution 30%过氧化氢溶液(30%双氧水) 500ml MS5603-50ML tert-Butyl hydroperoxide (TBHP) Solution叔丁基过氧化氢溶液 50ml MZ2401-100UL PMA (TPA, 5mg/ml)佛波肉荳蔻醋酸(5mg/ml) 100μl MX4455-1MG Dihydrorhodamine 123 (DHR 123)二氢罗丹明123 ...
3. 去除培养基,用HBSS清洗细胞2次,添加Photo-oxidation Resistant DCFH-DA Dye Working solution。 4. 37℃、5% CO2培养箱内培养30 min。 5. 去除培养基,用HBSS清洗细胞2次,添加Highly Sensitive DCFH-DA Dye Working solution。 6. 37℃、5% CO2培养箱内培养30 min。
1, H2DCFDA was dissolved into 10 mM DMSO stock solution and further diluted with PBS before use.2. Adherent cells are incubated with 5 µM H2DCFDA solution for 30 min at 37°C, protected from light, then harvested with 0.05% trypsin-EDTA solution, suspended in fresh medium and ...