The fluorescence intensity is proportional to the ROS levels within the cell cytosol. The effect of antioxidant or free radical compounds on DCF-DA can be measured against the fluorescence of the provided DCF standard. The kit has a DCF detection sensitivity limit of 10 pM. Each kit provides ...
where Ft30=fluorescence at time 30 min and Ft0=fluorescence at time 0 min. 不知道,这里的Ft0 指的是不是未加DCF前的读数? 如果是这样的话, 那这个Ft0数值会很低, 而Ft30会很高, 那么一相除就会很大影响? (为什么不直接相减救行了? wmp1234(2013-1-13 11:14:14) ...
three times for each treatment. Mean fluorescence intensity is quantified using software. Cells that had been incubated without DCFH-DA are used as negative control. The distribution of fluorescent dichlorofluorescein (DCF) on the cell monolayer is visualized and photographed using a fluorescence ...
Resuspend cells in PBS, seed in 96-well microplate with 100,000 stained cells/well and measure fluorescence immediately. *X represents the volume of DCFH-DA to obtain the optimal concentration related to the cell line used. NOTE: To create positive controls, oxidative activity is stimulated with...
where Ft30=fluorescence at time 30 min and Ft0=fluorescence at time 0 min. 不知道,这里的Ft0 指的是不是未加DCF前的读数? 如果是这样的话, 那这个Ft0数值会很低, 而Ft30会很高, 那么一相除就会很大影响? (为什么不直接相减救行了? 2541(2012-9-09 12:22:34) ...