The recruitment of locally enriched DNMT3A and the assistance of the co-facilitator DNMT3L, have been shown to enhance the catalytic efficiency of de novo DNA methylation [19,22]. Although our d9-D3A fusion construct demonstrated considerable methylation activity, to maintain more effective mCpG i...
a direct fusion of the C-terminal portion of DNMT3L to the catalytic domain of Dnmt3a (D3a3L) to a DNA targeting module (zinc finger protein or dCas9) enabled efficient DNA methylation at endogenous target sites [29,42,53]. We have shown that the...