Soluble in regular aqeous solution as well as most organic solvents; Copper-free Click reaction procedures: Prepare the azide-containing reagents in reaction buffer. Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reage...
DBCO (Dibenzocyclooctyne) is a commonly used copper free click chemistry reagent that can react with azide group with high specificity. Reaction between DBCO and azide allows fast and efficient attachment of desthiobiotin to target molecules or materials. Resulted desthiobiotin conjugate is able to ...
such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modif...
The reaction between DBCO and azides is blazingly fast, orders of magnitude exceeding the rate of copper-catalyzed reaction between azides and terminal alkynes. It is also catalyst-free. This reagent can be used to conjugate sulfo-Cyanine5.5 dye to a variety of azide-labeled molecules.结构...
The DBCO derivative of ATTO 425 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (...
力引发的的叠氮-炔基偶合反应(Strain-promoted Azide - Alkyne Click Chemistry reaction,SPAAC) 特点: 检测小分子叠氮化物比CuAAC反应更快 不需要铜离子,无 毒性 没有催化剂或者配体,因此无需大量的条件优化 适合与四氮 杂苯-反式环辛烯连接反应进行双标记方法 陕西新研博美生物科技有限公司是一家致力于...
Click Chemistry Ligand conjugationrequires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN:Tetrazine and TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites.Click here for a list of click chemistry modifications...
The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjμgation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO ...
Dibenzocyclooctyne (ADIBO, DBCO) is one of the most reactive cycloalkynes for strain-promoted alkyne azide cycloaddition (SPAAC) — a copper-free click chemistry reaction. DBCO reacts instantly with azides. The reaction rate is much higher than that of copper-catalyzed reaction, and reactions with...
Copper-free Click reaction procedures: Prepare the azide-containing reagents in reaction buffer. Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent. Incubate the reaction at room temperature for 2~4 hours or 2~...