The final melt curve was obtained by ramping from 68 to 98°C in 1°C steps every 5s. To determine primer efficiencies and optimal quantification thresholds, a dilution series of a cDNA mix consisting of 4 ul solution from every sample was created. Six 10-fold dilution steps were carried ...
The time-series of affected rice maps for the whole season were stacked to calculate a flood frequency map which was used to visually assess severely affected areas and flooding risk. The affected rice map was analyzed and evaluated mainly based on statistical reports from GSO for the provinces ...