Measurement unit of molecular mass (M r ), generally used to estimate the size of macromolecules, 1 Da = 1.661 × 10 24 g (1/12 of the MW of the C 12 isotope). 1 MDa is 10 6 Da. 1 pg of DNA is about 0.60205 × 10 12 Da; the M r of a nucleotide pair is about 650 Da...
[f. the name of John Dalton (see Daltonian a. and n.).]A name for the atomic mass unit (see atomic a. and n. A. 1), used chiefly in Biochem.; freq. used as a dimensionless unit of molecular weight.1938C. M. Beadnell Dict. Sci. Terms 65/1 Dalton, mass unit, being the 1...
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The molar volumes of all gases are the same when measured at the same temperature and pressure. But the molarmassesof different gases will vary. This means that different gases will have differentdensities(different masses per unit volume). If we know the molecular weight of a gas, we can c...
A system involves both reversible expansion work (–∫p dv) and reversible electrochemical work (∫ϕdq, where ϕ is the voltage and q is the charge per unit mass). For such a system, its specific enthalpy is now defined as h = u + pv –ϕq. a. Find an expression for the di...
The WAXD analysis assumed an orthorhombic unit cell commonly applied to β-sheet crystallites in semi-crystalline fibers72,73,74. In particular, the 1D radial intensity profile along the equator includes two main equatorial Bragg reflections. Here the innermost equatorial peak is indexed as (200),...
In all, 30 mL of cell lysis buffer (150 mM NaCl, 15 mM Tris, 1 mM MgCl2, 4 M urea, 0.2 mg/mL lysozyme, and 0.05 unit/mL DNase) were added to re-suspend the pellet and vigorously mixed for 1 h. Cell lysate was then sonicated with Branson Sonifier 450 for 2...
(Figures S2E and S2F) (Jiang et al., 2018; Mattevi et al., 1992), covering the top, external part of the trimeric asymmetric unit of the core (Figures S5G and S5H); and (2) inter-trimeric contacts mediated by helix G341–G359 of the homodimer (G531–G551 in human) (Figures S...
The final specific activity of the purified cathepsin-L1 proteinease was 11.67×103units/mg protein (where one unit releases 1 μmol of AMC/min). Samples from the purification were taken and loaded onto GS, SDS-non-reducing and SDS-reducing polyacrylamide gels (10% acrylamide, 0.1% SDS) in...
Purification after DTT reduction was accomplished by ultrafiltration in a Filtron Minisette unit. The Minisette was fitted with two Filtron 30K cassettes and was connected to a Watson Marlow 604S pump with Bioprene tubing. The MAb solution was ultrafiltered at 0° C. under Ar against Ar-bubble...