They can be used to assay IL-1 because they proliferate minimally in response to Con A in the absence of IL-1 or feeder cells. Supernatant media from Con A stimulated D10.G4.1 cells contain IL-4 and IL-5. Tested and found negative for ectromelia virus (mousepox). Propagation: ...
We describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1尾 or 伪 in the absence of mitogens. D10S cells have been maintained in culture for over two years without splenic cell feeder layers nor...
We describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1β or α in the absence of mitogens. D10S cells have been maintained in culture for over two years without splenic cell feeder layers nor...