将AAV-DIO-eYFP注射到D1-Cre小鼠的VTA中,可以观察到D1神经元轴突在mPFC、NAc、LS、BLA和vHipp中的分布(图6A),这表明VTA D1神经元可能以这些脑区为靶点来调节焦虑样行为。为了确定参与调节焦虑的VTA D1神经元的可能靶点,将ChR2...
Wild-type C57BL/6J and Sirt1loxp/loxp D1-Cre mice were used in this experiment, and Sirt1loxp/loxpD1-Cre(-) mice were used as a control for conditional knockout mice. Mice were divided into saline control and heroin-dependent groups. Behavioral methods were used to record the withdrawal...
品系描述 将Kozak-Cre-pA-pGK-Neo插入到小鼠Foxd1基因起始密码子处。 *使用本品系发表的文献需注明: Foxd1-Cre-pGK-Neo mice (Cat. NO. NM-KI-241417) were purchased from Shanghai Model Organisms Center, Inc.. 鉴定方案 下载 相关品系Foxd1-Flox...
These mice carry a H133N mutation of Gpld1 gene. *使用本品系发表的文献需注明: Gpld1-H133N mice (Cat. NO. NM-KI-210088) were purchased from Shanghai Model Organisms Center, Inc.. 相关品系 Gpld1-H133N 品系名:C57BL/6Smoc-Gpld1em1(H133N)Smoc 品系状态:胚胎冻存 | 目录号:NM-KI...
To test whether activation of NAcD1R neurons is sufficient to restore states of consciousness during the sevoflurane maintenance phase, we injected AAV-DIO-ChR2-mCherry or AAV-DIO-mCherry into the NAc of D1R-Cre mice (Figure 4A). Functional expression of ChR2 was confirmed by whole-cell pat...
A Effects of BDNF knockdown on N2O-induced CPP behaviors in mice (n = 9 / group). B Effect of BDNF knockdown on the expression of VTA’s BDNF mRNA (n = 3 / group). C The schematic of rAAV-hSyn-NLS-Cre-P2A-mCherry microinjection in VTA. D–G Effect of BDNF knockdown...
Mice were bilaterally infected at P21 by stereotactic injections of AAVs expressing ΔCre-eGFP (control) or Cre-eGFP, and subiculum neurons were analysed in acute slices 2-3 weeks later. In this and all following experiments, ΔCre refers to the expression of a mutant inactive Cre that is ...
(1 µg/mL) selection, successfully targeted subclones were isolated49. Finally, the puromycin resistance cassette was removed by the transient expression of Cre recombinase50(Supplemental Fig.3B). To induce the myogenic differentiation of hiPSCs, these cells were used to generate Dox-inducibleMYOD...
To determine the effect of NeuroD1 knockout in these tissues, a Cre/loxP recombination strategy was used to target a NeuroD1 floxed gene and generate NeuroD1 conditional knockout (cKO) mice. Tissue specificity was conferred using Cre recombinase expressed under the control of the promoter of ...
However, it is unknown whether the same neuronal populations are targeted in GFP and Cre mice. Additionally, while D1Rs and D2Rs are known to be expressed in different striatal neurons, their expression patterns outside the striatum remain unclear. The present study addressed these two questions...