L-arabinose isomerase by rational design to enhance galactose isomerisation activity. In silico molecular docking, analysis has revealed that F279 is sterically hindering the binding of D-galactose at the C6 position. Substitution of bulky Phe residue with smaller hydrophilic residues such as Asn and...
We purified recombinant glucose-6-phosphate isomerase from Pyrococcus furiosus using heat treatment and Hi-Trap anion-exchange chromatography with a final ... Ran-Young,Yoon,Soo-Jin,... - 《Applied Microbiology & Biotechnology》 被引量: 25发表: 2009年 加载更多研究...
d-Tagatose was continuously produced using thermostable l-arabinose isomerase immobilized in alginate with d-galactose solution in a packed-bed bioreactor. Bead size, L/D (length/diameter) of reactor, dilution rate, total loaded enzyme a... SA Ryu,SK Chang,HJ Kim,... - 《Biotechnology Progres...
LIANG M,CHEN M,LIU X,et al.Bioconversion of D-galactose to D-tagatose:continuous paked bed reaction with an immobilized thermostable L-arabinose isomerase and efficient purification by selective microbial degradation[J].Applied Microbiology and Biotechnology,2012,93(4):1469-1474....
Improving Catalytic Efficiency of L-Arabinose Isomerase from Lactobacillus plantarum CY6 towards D-Galactose by Molecular Modification L-Arabinose isomerase (L-AI) has been commonly used as an efficient biocatalyst to produce D-tagatose via the isomerization of D-galactose. However, it rem... Lu, ...
An efficient method for biotransformation of D-galactose to D-tagatosewas achieved by a whole-cell L-arabinose isomerase reaction of Acidothermuscellulolytics ATCC 43068 for the first time. It was observed that the straingrew well on the... L Cheng,MU W.,B Jiang - 《Journal of Food Biochemi...
L-arabinose isomeraseD-tagatoseMutationSubstrate specificityL-arabinose isomerase is an enzyme converting D-galactose to D-tagatose. D-tagatose is a potential sweetener-sucrose substitute which has low calorie. This research was to clone and sequence araA gene from marine bacterial strain Geobacillus ...
L-ARABINOSE ISOMERASE FOR CONVERTING D-GALACTOSE INTO D-TAGATOSE IN A DAIRY PRODUCT WHICH CONTAINS D-GALACTOSEMoez RHIMIHichem CHOUAYEKHEmmanuelle MAGUINSamir BEJAR
Structurally, LacAB belongs to the RpiB/LacAB superfamily, having a Rossmann-like αβα sandwich fold as has been identified in pentose phosphate isomerase and hexose phosphate isomerase. In contrast to other family members, the LacB subunit also has a unique α7 helix in its C-terminus. ...
L-ARABINOSE ISOMERASE FOR CONVERTING D-GALACTOSE INTO D-TAGATOSE IN A DAIRY PRODUCT WHICH CONTAINS D-GALACTOSERhimi, MoezChouayekh, HichemMaguin, EmmanuelleBejar, Samir