Finally, it has been confirmed by heat inactivation that there is no interference between specific assays of altronate and mannonate oxidoreductases. Compounds such as NADH, NADPH, altronate and mannonate protect enzymatic activity from degradation by heat, whereas NAD + , tagaturonate and fruct...
La D‐mannonate: NAD‐oxydoréductase d'Escherichia coli K12 D-Altronate: NAD-oxidoreductase of Escherichia coli K12 has been purified. The purification consists of four steps: nucleic acids precipitation with protamine sulfate; ammonium sulfate precipitation; ion exchange chromatography on DEAE-c... ...
d-mannonate:NAD oxidoreductase (d-mannonate:NAD+ 5-oxidoreductase, EC 1.1.1.57) from Escherichia coli normally catalyzes the reaction: d-fructuronate + NADH ⇌ d-mannonate + NAD+ [4,5]. It can also catalyze the reduction of d-glucuronate according to the equation: d-glucuronate + NADH ...
Dead-end product inhibition studies with fructuronate, mannonate and ATP, and binding studies in relation to the protection of the enzyme against thermal inactivation, are consistent with an ordered bi-bi enzymic mechanism for the altronate oxydoreductase. The occurrence of two possible enzymic forms...
Calorimetric estimations of aldonate oxidoreductases from Escherichia coli K 12 : Applications Altronate (EC 1.1.1.58) and mannonate oxidoreductases (EC 1.1.1.57), two NAD + enzymes involved in the hexuronate pathway of E. coli K 12, respectively catalyze the reversible dehydrogenation of altron...