Repetitive cycles of refinement were done in COOT (34) and Phenix refine (35) to obtain final structures (Tables S1 and S2). Structures were validated using the Molprobity server and all figures were rendered using PyMoL. Kinetics and inhibition assays To measure DXPS activity and kinetic ...
A 10-primer multiplex PCR-based method has been developed for determining the presence of RHD exon 7 and intron 4, the 37 bp insert in RHDψ exon 4, and the RHCE C andc alleles. The primers and their concentrations are shown in Table 2. Thirty cycles of PCR were performed at 94°...
Combing and brushing of hair mechanically abrades the fibre cuticle making this rougher and increasing the frictional characteristics. Hair lightening, such as bleaching, or colouring treatments generally involve an oxidative step to break down melanin and develop the new hair colour, but these processe...
Increasingly, the intrinsic and extrinsic motivations to use systems are intertwined, meaning that many IS are used to fulfill a mixed range of needs and wants, which has led to the emergence of studies on dual-purposed IS (Verhagen et al.2012; Wu and Lu2013). Dual-purposed systems are ...
As a scholar of Riggleman Manor Archives, in good standing with the scientific and religious communities of Warrington. I, Nicodemus Pandit would like to present this general summary of the religions of Pillar and Bolster Heart to the community at large. I do this in order to bring about a ...
Cardiac measurements were performed off-line from the M-mode images using a leading edge-to-leading edge technique according to the American Society of Echocardiography guidelines.20 Measurements from five cardiac cycles were averaged to obtain the following parameters in diastole and systole: ...
The initial model was subjected to 20 cycles of rigid-body refinement in the 10.0–3.0 å resolution range, lowering the R factor and Rfree to 41.0% and 47.3%, respectively. Subsequently, a series of atomic coordinates restrained refinement cycles was carried out, alternated with rebuilding ...
The cells were transfected with plasmids: 1.3 pmol psPAX2 and 0.72 pmol pMD2.G (Addgene plasmids #12260 and #12259; both were gifts from Didier Trono) and 1.64 pmol respective pLKO plasmid using PEI MAX 40K (Polysciences, Warrington, PA, USA) at a ratio of DNA to PEI 1:3. Medium ...
The experiment began in October 2017 and was carried out for 21 months at a commercial mandarin orchard located in Torre Pacheco, Murcia, Spain (37°47′30″ N; 1°03′85″ W; 30 m above sea level). The experiment finished by mid-June 2019 and covered two crop cycles. ...
(Polysciences, Inc., Warrington, PA, USA) for 12 h, and polymerized in an electron microscope oven at 65 °C for 12 h. The resulting block was sectioned into 200 nm slices, using a diamond knife, on an ultramicrotome and stained with toluidine blue for light microscopy. These sections ...