The invention relates to an assay for determining NK-Cell cytotoxicity and the invention particularly relates to a new and improved "whole blood flow cytometry "based assay. Since in this method, whole blood is used instead of purified cells, the requirement of blood sample volume is ...
Cell viability can be detected through various mechanisms, such as membrane integrity, enzyme activity, or metabolic activity. Each viability reagent forms the basis of an assay to evaluate cell heath through a single-parameter readout or using multiple measures of detection—viability kits. Additional...
Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium...
Vizler C, Nagy T, Kusz E, Glavinas H, Duda E (2002) Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity . Cytometry 47 : 158–162Vizler C, Nagy T, Kusz E, Glavinas H, Duba E. Flow cytometric cytotoxicity assay for measuring mammalian and avian NK ...
This assay detects and quantifies dead target cells using flowcytometry. With our method, living and dead target and effector cells can be distin- guished based on differential staining by fluorescent dyes. Moreover, this assay is capable of assessing the cytotox- icity induced by frozen ...
Flow cytometry provides a rapid and reliable method to quantify the live and dead cells in the cell suspension. This assay is dependent on the fluorescence intensity of the PI dye, which is intercalating between the DNA base pairs. PI dye uptake assay is commonly used for identifying dead cell...
Cell viability can be detected through various mechanisms, such as membrane integrity, enzyme activity, or metabolic activity. Each viability reagent forms the basis of an assay to evaluate cell heath through a single-parameter readout or using multiple measures of detection—viability kits. Additional...
Each viability reagent forms the basis of an assay to evaluate cell heath through a single-parameter readout or using multiple measures of detection—viability kits. Additionally, ready-to-use options are available for your everyday experimental needs. Viability assays are used to evaluate the ...
Cytotoxicity and antibody binding by flow cytometry: A single assay to simultaneously assess two parameters Background: Cell death, as determined by complement-dependent cytotoxicity or antibody binding, as assessed by flow cytometry, are the two main methods his... CRH Saw - 《Cytometry Part B ...
Piriou L, Chilmonczyk S, Genetet N, Albina E (2000) Design of a flow cytometric assay for the determination of natural killer and cytotoxic T lymphocyte activity in human and in different animal species. Cytometry 41:289–297 CAS PubMed Google Scholar Download references ...