RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook lymphocytotoxicity [lim″fo-si″to-tok-sis´ĭ-te] the quality or capability of lysing lymphocytes, as in procedures in which lymphocytes having a specific cell surface antigen are lysed when incubated with ant...
CYTOTOXICITY ASSAYThe present invention relates to methods for detecting CTL activity using flow cytometry to detect cleavage of cytotoxicity-related enzymes.P·邓L·何L·拉德万伊D·萨尔哈
Different approaches for evaluating effector cell lytic capacity or target cell lysis were developed (Fig. 1). Lytic potential of effector cells is assessed by flow cytometry by the measure of LAMP-1 externalization (CD107a assay) that comes out as a surrogate marker of cytotoxic potential of ...
Cell viability can be detected through various mechanisms, such as membrane integrity, enzyme activity, or metabolic activity. Each viability reagent forms the basis of an assay to evaluate cell heath through a single-parameter readout or using multiple measures of detection—viability kits. Additional...
This assay detects and quantifies dead target cells using flowcytometry. With our method, living and dead target and effector cells can be distin- guished based on differential staining by fluorescent dyes. Moreover, this assay is capable of assessing the cytotox- icity induced by frozen ...
Cell viability can be detected through various mechanisms, such as membrane integrity, enzyme activity, or metabolic activity. Each viability reagent forms the basis of an assay to evaluate cell heath through a single-parameter readout or using multiple measures of detection—viability kits. Additional...
Vizler C, Nagy T, Kusz E, Glavinas H, Duda E (2002) Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity . Cytometry 47 : 158–162Vizler C, Nagy T, Kusz E, Glavinas H, Duba E. Flow cytometric cytotoxicity assay for measuring mammalian and avian NK ...
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[1]. however, the cdc assay detects only complement fixing antibodies and there are weaknesses associated with this assay such as insensitivity and non-specific responses. dsa can be detected with higher sensitivity by using the flow cytometry based crossmatch (fcxm) assay [7]. importantly, low ...
Cytotoxicity MTT Assay is a colorimetric assay which measures cell viability, proliferation and cytotoxicity.