【工具】Cytotoxicity Assay(细胞毒性测试) Cytotoxicity Assay的方法有很多,今天给大家介绍的是通过测量LDH浓度的方法进行的assay,用的kit是来自于Promega的CytoTox 96 Non-radioactive。 LDH的测量机理如上图: 细胞死亡的时候LDH(一种酶)会漏出来,使得NAD+变成NADH。 而NADH刚好是INT转换到Formazan的必需品。 formazan...
Enzymatic cytotoxicity assays Lactate dehydrogenase (LDH) and glucose 6-phosphate dehydrogenase (G6PD) are enzymes present in many different cell types. When the cellular plasma membrane is damaged, LDH and G6PD will be released in the cell culture medium. CyQUANT Cytotoxicity Assay Kits are colorimet...
Lactate dehydrogenase (LDH) and glucose 6-phosphate dehydrogenase (G6PD) are enzymes present in many different cell types. When the cellular plasma membrane is damaged, LDH and G6PD will be released in the cell culture medium. CyQUANT Cytotoxicity Assay Kits are colorimetric- or fluorescence-based ...
Synonyms cytotoxicity assay , natural killer (NK) cell assay, cytotoxic T lymphocyte (CTL) assay , chromium release assay Short Description Cytolytic activity is a property of specialized immune cells, such as natural killer (NK) cells and cytotoxic T lymphocytes , enabling surveillance and ultimate...
乳酸脱氢酶细胞毒性检测试剂盒(LDH Cytotoxicity Assay Kit),也称乳酸脱氢酶检测试剂盒(LDH Assay Kit)或乳酸脱氢酶释放检测试剂盒(LDH Release Assay Kit),是一种基于Diaphorase催化INT的显色反应,通过比色法检测细胞毒性时释放的乳酸脱氢酶活性或检测其它样品中的乳酸脱氢酶活性的试剂盒。
乳酸脱氢酶细胞毒性检测试剂盒(LDH Cytotoxicity Assay Kit),也称乳酸脱氢酶检测试剂盒(LDH Assay Kit)或乳酸脱氢酶释放检测试剂盒(LDH Release Assay Kit),是一种基于diaphorase催化的INT显色反应,通过比色法检测细胞毒性时释放的乳酸脱氢酶活性或检测其它样品中的乳酸脱氢酶活性的试剂盒。
The Incucyte®Live-Cell Analysis System enables real-time, automated cytotoxicity assays within your tissue culture incubator. The Incucyte®Cytotoxicity Assay uses Incucyte®Cytotox Dyes to make real-time measurements of cell death based on cell membrane integrity. ...
Application note: Delfia cell proliferation assay on the SpectraMax i3 Multi-Mode Microplate Reader Colorimetric cell viability, cell proliferation, cytotoxicity assays (MTT, XTT, MTS) Colorimetric assays using tetrazolium salts like MTT, XTT, and MTS can be used to measure cell proliferation and cyto...
Lactate Dehydrogenase Cytotoxicity Assay Kit Origin Animal/Rabbit Shipping & Storage Information Storage -20°C Shipping Wet ice in continental US; may vary elsewhere Stability ≥ 1 year Certificates of Analysis & Batch Specific Data Get Batch-Specific Data and Documents by Batch Number Provide batch...