However, given extensive cell-to-cell variation, these data cannot be readily assembled into three- or four-dimensional maps of protein concentration that can be compared across different cells and conditions. We have developed a method to enable comparison of imaging data from many cells and ...
Compared to erythrocytes resealed in the presence of 1 mM ATP (the approximate normal physiological ATP concentration of erythrocytes), erythrocytes resealed with 10 mM AMP-PNP were inhibited from supporting merozoite invasion by ~60% (p < 0.0001, two-tailed unpaired t-test). Under...
Compared with ‘Ca.P. syntrophicum’, the genome of Loki-B35 is significantly larger (by approximately 1.6 Mb), which is also reflected by 2,256 unique proteins (Fig.2b). Functional annotation of orthogroups revealed that genes of Loki-B35 are enriched in almost all categories, reflecting ...
The presence of βII-tubulin at the outer mitochondrial membrane explains the very high value of apparent Km for free exogenous ADP in adult permeabilized cardiomyocytes which is an order of magnitude higher than in isolated mitochondria (370 μM compared to 10–15 μM, respectively) [8], [20...
To begin, the SWATH-MS data obtained from the cortical neurons was compared to a reference data set derived from a model of cell membrane injury (Sønder et al., 2019) associated with pronounced membrane- and cytoskeleton-related changes. Twelve proteins were found to be significantly changed ...
Since hypoxia influences actin dynamics, it is a straightforward finding that cell shape, adhesion, and movement are greatly altered in hypoxic cells compared to cells grown in normoxia.3,4 However, depending on the origin of the cells and the experimental setup, the literature shows at least ...
RNA was detected by real time RT-PCR. Expression of PGK or GLUT-1 in GFP cells was set to 1 in each experiment. Data are means ± SD of 4 to 9 experiments. *** p < 0.001, ** p < 0.01 compared to shGFP cells, ANOVA with Tukey’s multiple comparison test. (C) ...
Janmey PA, Euteneuer U, Traub P, Schliwa M (1991) Viscoelastic properties of vimentin compared with other filamentous biopolymer networks. J Cell Biol 113(1):155–160 Google Scholar Janson ME, Dogterom M (2004) Scaling of microtubule force‐velocity curves obtained at different tubulin concentr...
1c), and furthermore, the mean length of cellular synapses in the stiff group were longer compared to those in the soft group (Fig. 1d). Fig. 1 Cell morphology changes of chondrocytes in response to PDMS substrates with different stiffnesses. a Representative SEM images showing the cell ...
initiation was observed. Compared to WTXcc, F-actin seeds were less-stimulated at 12 hpi and less-inhibited at 24 hpi inXccΔxopR-infected seedlings (Fig.1f, g), suggesting that XopR hijacks actin nucleation. OtherXccvirulence factors might also participate, additively or synergistically, ...