Furthermore, we show that FIP3 binds to Cyk-4 at late telophase and that centralspindlin may be required for FIP3 recruitment to the mid-body. We have mapped the FIP3-binding region on Cyk-4 and show that it overlaps with the ECT2-binding domain. Finally, we demonstrate that FIP3 ...
FIP3 binds to CYK-4 at late telophase, and for its recruitment in the midbody centralspindlin complex is required. The FIP3-binding region on CYK-4 overlaps with the ECT2-binding domain. FIP3 and ECT2 form exclusive complexes with CYK-4 and removal of ECT2 from the centraspindlin ...
andBRAFV600E-expressing (+Dox) RPE-1 cells treated with NSC2366 or EHT1864. Drugs were added coincident with Dox administration. Images are maximum intensity projections of z-stacks (0.20 µM). Scale bar = 7.5 µM.EMean RHOA fluorescence intensity at the equator...
However, intensity differences between the lowest and highest intensities at metaphase, anaphase and telophase were not significantly different in dlg;;pins double mutants compared with wild type (Fig. 7d). We independently measured cleavage furrow positioning at the onset of furrowing in wild-type, ...