Patrick Stordeur,Lionel F Poulin,Ligia Craciun,et al.Cytokine mRNA quantification by real-time PCR. Journal of Immunological Methods . 2002P. Stordeur, L.F. Poulin, L. Craciun, L. Zhou, L. Schandene, A. de Lavareille, S. Goriely, M. Goldman, Cytokine mRNA quantification by real-...
Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis Fluorescent data obtained from real-time PCR must be processed by some method of data analysis to obtain the relative quantity of target mRNA. The method c... T Iko,A Bukovská,J Koppel - ...
A whole blood assay was developed for T-lymphocyte analysis which allows the quantification of induced cytokine mRNA expression. We applied a novel kinetic... HÄRTEL,BEIN,KIRCHNER,... - 《Scandinavian Journal of Immunology》 被引量: 72发表: 1999年 Quantitative Real-Time RT-PCR Using Hybridizat...
(F) Quantitative real-time PCR data for MYC mRNA in MCF-7 cells treated with Veh, IL1β, or a combination of IL1β and IKK7. Values are expressed as mean ± SEM. ∗p < 0.05 (Student’s t test), IL1β + IKK7 compared with IL1β sample. (G) Same as (F), but cells wer...
Quantitative real-time PCR for mRNA Total RNA was extracted from cells using Qiagen RNeasy Mini Kit (Qiagen, Manchester, UK) according to the manufacturer's protocol. RNA was reverse transcribed using random hexamers (Life Technologies Ltd) and the Reverse Transcriptase Kit SuperScript III (Life ...
After retrieving the beads, the hybridized mRNA was reverse-transcribed into complementary DNA (cDNA). The resulting cDNA was amplified by a minimal number of PCR steps, fragmented, ligated to adaptors and PCR amplified to construct a sequencing library. The gene expression libraries generated were ...
The objectives of the current study were: to evaluate the role of serum cytokines levels (TNF-α, INF-γ, IL-2, IL-4, and IL-10) and mRNA expression by real-time PCR, compare these two methods and examine Th-1, mature Th-0, and Th-2 cytokine profiles. Therefore, a cross-...
Quantification of viral RNA load by real time PCR To confirm the above results, the level of viral RNA was measured by real time PCR at all time points of EV-A71 infection in both the cell lines. The kinetics of viral RNA in response to the three genotypes in SK-N-SH cells did not...
Samples were assayed in triplicate on a 384 well plate using the 7900 Fast Real-Time PCR system (Applied Biosystems). The widely-accepted comparative Ct (threshold cycle) method was used to perform relative quantification of qPCR results [37]. An arithmetic formula (fold differ- ence = 2-Δ...
The multiprobe ribonuclease protection assay (RPA) is a highly sensitive and specific method for simultaneous detection and quantification of several species of mRNA. Three most distinct advantages of the multiprobe RPA method are: high sensitivity and specificity, capacity to simultaneously quantify more...