Fast inhibitory neurotransmission is essential for nervous system function and is mediated by binding of inhibitory neurotransmitters to receptors of the Cys-loop family embedded in the membranes of neurons. Neurotransmitter binding triggers a conformati
This solution was loaded onto a phenyl-Sepharose high performance (Low Sub) column (GE Healthcare) and eluted with a 600-ml linear gradient to buffer without ammonium sulfate. The fractions corresponding to the Prx molecule, as determined by SDS-PAGE, were dialyzed into 20 mm Tris, pH 7.9,...
Lastly, the PCOS Quality of Life instrument (PCOSQ) was a useful screening tool, correlating well with PCOS physical and emotional symptoms. Although the PCOSQ emotions domain correlated well with each of the nine subscales and three global indices of the BSI [range (r = -.40 through -....
with buffer B, the bound proteins were eluted by a linear gradient of 0.05–0.83 M NaCl. The proteins were dialyzed against buffer B, and then loaded onto a HiTrap heparin column (GE Healthcare). After washing the column with buffer B, the proteins were eluted by a linear gradient of 0....