The human cytochrome P450 (CYP) enzyme CYP4A22 is an orphan CYP with unknown function. Here, through functional expression in fission yeast, we show that CYP4A22 catalyzes fatty acid hydroxylation as well as aliphatic or aromatic hydroxylations of luciferin‐based probe substrates. Mechanistic ...
CYP4A22 activity is inhibited by three unspecific CYP inhibitors. A comparison of CYP4A22*1 (the reference standard sequence) with CYP4A22‐WT (the most common allele) revealed that for the four substrates tested the WT‐enzyme always had lower activity.Pradeepraj Durairaj...