a toxic antibiotic that inhibits protein synthesis, used as a fungicide in agriculture Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 2009, 2011, 2014 ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...
CHX enjoys maximum popularity in biomedical research as a mechanistic tool to modulate protein synthesis and is frequently used to determine the half-life of protein in any cellular system. LDdoses for orally administered CHX for rats and mice are 2 and 133 mg kg, respectively....
the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
(0hourofCHXtreatment).CandD,thehalf-lifeofDrp1inthepresenceofGFP-ParkinorGFPalonewasanalyzedby[35S]methioninepulse-chaseassay,andtheproteinlevelswerequantified.FigureS2.InvitroubiquitinationofDrp1byMARCH5.InvitrotranslatedDrp1wasincubatedwithpurifiedubiquitin,E1,E2(UbcH7),variousParkinproteinsand...
Golgienzymestypically have a half-life of over 20h or more; thus, the fluorescence intensities should be minimally affected by degradation of the FP-tagged Golgi enzymes. However, if the protein of interest has a shorter lifetime, thenprotein degradationcan change the FP signal significantly. This...
Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein (enzyme). Treating cells with cycloheximide in a time-course experiment followed by Western blotting of the cell lysates for the protein of interest can show differences in protein ...
CHX enjoys maximum popularity in biomedical research as a mechanistic tool to modulate protein synthesis and is frequently used to determine the half-life of protein in any cellular system. LDdoses for orally administered CHX for rats and mice are 2 and 133 mg kg, respectively. 展开 关键词:...
Cycloheximide is used to study protein synthesis, expression and degradation, and determine the half-life of proteins. Mechanism of Action Cycloheximide binds to the ribosome and inhibits the eEF2-mediated translocation step in protein synthesis, thus blocking translational elongation. Spectrum ...
The SV40-transformed system could be pertinent to the question of whether DNA synthesis inhibition depends solely on the synthesis of some unknown protein(s) of short half life, in some way necessary for the polymerization reaction, or addition- ally on the levels of newly synthesized precursors....