Cycloheximide (CHX) treatment of myotubes results in a 7-fold increase in alternative Actg1.Meghan C. DrummondKaren H. Friderici
蛋白合成抑制剂亚胺环己酮可以诱导小鼠卵母细胞发生孤雌活化 ,但其机制尚未完全阐明。 2. The expressions of MEPE / OF45 gene fragments were observed in the cells after cycloheximide(CHX) treatment to inhibite protein synthesis. 方法:选取人宫颈癌细胞HeLa为靶细胞,从中提取总RNA,设计扩增人MEPE/OF45...
To gain an additional insight into the role of AD and CHX in the ligand-dependent and ligand-independent turnover of AHR, studies were carried out in cell lines from different species. The results showed that the treatment of rat smooth muscle cells (A7), mouse fibroblasts (10T1/2), or ...
(100 μg/ml) for the indicated hours, and Western blotting was performed. B, the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP ...
(0hourofCHXtreatment).CandD,thehalf-lifeofDrp1inthepresenceofGFP-ParkinorGFPalonewasanalyzedby[35S]methioninepulse-chaseassay,andtheproteinlevelswerequantified.FigureS2.InvitroubiquitinationofDrp1byMARCH5.InvitrotranslatedDrp1wasincubatedwithpurifiedubiquitin,E1,E2(UbcH7),variousParkinproteinsand...
Exposed embryos had only reached the 4-cell stage, while a significant proportion of the eggs treated with 0.1μM CHX or no CHX (i.e., control) had attained the 8-cell stage. Treatment of fertilized eggs with the transcriptional inhibitor actinomycin D (AMD) was utilized to demonstrate ...
Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was performed. B, the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the...
Cycloheximide_抗生素,真核生物蛋白质合成抑制剂_66-81-9_Apexbio Cycloheximide|Antibiotic,inhibiter of protein synthesis in eukaryotes|CAS# 66-81-9 抗生素，真核生物蛋白质合成抑制剂 客户使用Apexbio产品发表的文献 质量控制 质量控制和MSDS View current batch:3 3 Purity = 98.20% COA (Certificate Of ...
To determine whether CHX-induced apoptosis was mediated by a Fas-associated death domain (FADD)-dependent mechanism, a FADD-DN protein was expressed in cells prior to CHX treatment. Its expression effectively inhibited CHX-induced cell death, suggesting that CHX-mediated apoptosis primarily involves ...
CHX treatment did not significantly affect the distribution of PPO mRNA and PPO protein in explant tissues, but their levels were significantly lower than those of the untreated control.Previous article in issue Next article in issue Keywords Phalaenopsis PPO explant browning CHX gene expression...