the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...
FigureS1.Cycloheximide(CHX)chaseassayand[ 35 S]methioninepulse-chaseassayfor thehalf-lifeofDrp1.A,293TcellsweretransfectedwithGFP-ParkinandGFP-Parkin(ΔR2) for24hours.CellswerethentreatedwithCHX(100μg/ml)fortheindicatedhours,and Westernblottingwasperformed.B,thelevelofremainingDrp1atdifferenttimepoints...