W. Stacey, Variations in cyclin d1 levels through the cell cycle determine the proliferative fate of a cell, Cell Div., 1 (2006), 32.Yang K, Hitomi M, Stacey DW. Variations in cyclin D1 levels through the cell cycle determine the proliferative fate of a cell. Cell Div. 2006;1:32....
The corresponding relative mRNA levels were determined by quantitative reverse-transcriptase PCR (qRT-PCR) (Figure S2D). It seemed that USP27 was also transcriptionally regulated in the cell cycle progression (Figure S2D). Interestingly, USP27 expression might also be regulated by Fbxw7, a well-...
Cyclin A is expressed at particularly high levels, whereas cyclin E is virtually absent in the hematopoietic lineage [29]. Therefore, cyclin A–Cdk complex predominates over cyclin E–Cdk complex in HSCs to a great extent, and this renders these cells dependent on cyclin A for cell-cycle ...
Moreover, WNT signaling upregulates PROX1 expression in GCPs. These findings suggest that WNT-PROX1-CCND1-ATOH1 signaling cascade cooperatively controls proliferative and immature states of GCPs. We revealed that the express...
URG4, a novel oncogene located on the short arm of chromosome 7p13, promotes cell proliferation via the MAPK, PI3K, Akt, and NF-kappa B pathways. Studies have shown that high levels of URG4 protein have been found in many solid tumors and are involved in tumorigenesis and tumor ...
In this manner, G1 cyclins may be viewed as sensors to growth factor stimulation [5,8]. After a cell enters S, cyclin E is degraded, and CDK 2 forms complexes with cyclin A. Passage through the G1/S transition may be a result of increasing levels of cyclin A/CDK 2 complex. Cyclins...
Clinical trials with pan-CDK inhibitors, such as flavopiridol, have demonstrated low levels of clinical activity and drug target selectivity. The reasons for their failure in the clinic include the absence of clear biomarkers for response and the lack of a clear therapeutic window. The selective CD...
Cell cycle distribution was analyzed by the flow cytometry. Antisense technology was used to inhibit the expression of endogenous ClC-3 and Cyclin D1. RT-PCR detected the expression levels of destination gene. Results 40μg/ml ClC-3 antisense oligonucleotides suppressed obviously CNE-2Z cell ...
Moreover, WNT signaling upregulates PROX1 expression in GCPs. These findings suggest that WNT-PROX1-CCND1-ATOH1 signaling cascade cooperatively controls proliferative and immature states of GCPs. We revealed that the expression and phosphorylation levels of these molecules dynamically change during cerebe...
(p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme; tyrosine dephosphorylation, occurring at the onset of mitosis...