cyclic nucleotide dependent protein kinase and the phosphorylation of a single endogenous protein have been demonstrated in rod outer segment preparations (Lollyet al.,1977;Farberet al.,1979). Whether these changes in membrane permeability are related to visual transduction or light-dark adaptation are...
At the core of the GacS network is the unorthodox SK, GacS, which controls the phosphorylation of its output RR, GacA. In addition, there are several hybrid SKs that feed into this network, although by virtue of being the only SK in this network with a Hpt domain, GacS is the only ...
Identification and quantitation of signal molecule-dependent protein phosphorylation. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols; Gehring, C... A Groen,L Thomas,K Lilley,... - 《Methods in Molecular Biology》 被引量: 19发表: 2013年 Identification and Quantitation of Signal Mol...
Western blot analysis of phosphotyrosine expression in caudal and ejaculated populations of tammar spermatozoa revealed that elevation of intracellular cAMP levels, but not exposure to oxidants or NADPH, induced a dramatic increase in the overall level of tyrosine phosphorylation. Washed, ejaculated ...
a bell-shaped response curve concerning receptor activation, measured in terms of the relation between phosphorylation levels of the SH2 domains and VEGF concentration17,18. This implies that VEGFR activation increases as VEGF concentration rises until a threshold concentration is reached, maximizing the...
transferring phosphate to the latters’ serine or threonine residues. Upon phosphorylation, the protein substrates may act on the ion channels or they may become inhibited. Activated PKA may also act on (i.e. phosphorylate) specific proteins bound to the promoter regions of DNA, and thereby furt...
et al. Cilostazol suppresses angiotensin II-induced vasoconstriction via protein kinase A-mediated phosphorylation of the transient receptor potential canonical 6 channel. Arterioscler. Thromb. Vasc. Biol. 31, 2278–2286 (2011). Article CAS PubMed Google Scholar Puxeddu, E. et al. Interaction of...
VEGF receptor phosphorylation on stretched EC was assayed by immunoblotting.The steady-state level of VEGF mRNA in stretched VSMC increased 3.3 (+/- 0.6) fold above that of unstretched VSMC (p < 0.005). Migration of EC was stimulated 8.3 (+/- 1.1) and 14.6 (+/- 1.3) fold by media...
which exits through adenosine transporters to the subretinal space. Elevated extracellular adenosine enhances intracellular cAMP level via A2 receptors in cones but not in RPE. Extracellular dopamine levels are low. Elevated cone cytoplasmic cAMP triggers phosphorylation of regulatory proteins that activate ...
Systematic profiling of TCR signaling networks at single-cell resolution has been technically challenging due to the limited abundance of phosphoproteins in T lymphocytes and their small size (a T cell is 23 times smaller than a HeLa cell, on average). Most of the phosphorylation sites on TCR...