To address this question, we defined the changes in isoform use and polyadenylation site selection that occur as cells transition from proliferation to quiescence. We found that APA factors are expressed at lower levels as fibroblasts become quiescent, and that knockdown of these factors results in ...
A gain-of-function (GOF) mutant protein of p43Ntf6 was made as for the LOFs except the mutated site was from a glutamate at position 328 to asparagine, corresponding to the Drosophila sevenmaker GOF mutant, D334N (11). The GOF mutant protein was called p43Ntf6-E328N. Plasmid ...