Microscope The strains CCY9703 and CCY9710 were also slides were prepared by covering them with a thin cultured in liquid medium. Three liquid cultures of layer of 1% (w/v) molten agarose (50 1C; Sigma- each strain were grown under white light conditions Aldrich, Zwijndrecht, The ...
Differentiation of cells into akinetes was recognized by visual inspection under a Zeiss dissecting microscope (Carl Zeiss MicroImaging GmbH, Jena, Germany; Â 40 magnification) and defined on the basis of cellular dimensions (akinetes have larger diameter) and shape as previously described (Sukenik...
Preserved samples were loaded on a Palmer counting cell and counted using a compound microscope (Nikon Eclipse 80i) at ×400 magnification. A minimum of eight frames (volume 2.4 × 10−5 mL per frame) and 400 cells were counted to calculate the cell density. The cell density was con...
Northern hybridization was carried out after separation and blotting of 1 μg total RNA with a 32P-labeled, single-stranded transcript probe specifically recognizing atpT. (B) atpT mRNA accumulation after 6 h under the indicated conditions and treatments. CCCP and DBMIB were added to final ...
We used a Zeiss AxioImager A1 fluorescence microscope equipped with Nomarski optics and a Zeiss Colibri laser source. Sample injection. The suspension of live cells was aerosolized with helium in a gas dynamic nebulizer27, and delivered into the pulse train of the LCLS through an aerodynamic lens...
Cyanobacteria cells are frequently found and recognized using a microscope. A standard method for locating and identifying cyanobacteria cells is light microscopy. The sample is viewed under a microscope when a light source and the cells illuminate. Cyanobacteria cells may be recognized by their distinc...
The samples were stained with 5 µg/mL Hoechst 33342 (Thermo Fisher Scientific, Waltham, MA, USA) for 30 min and observed using 40× objective and 1.6× optovar under Zeiss Axio Observer Z1 microscope coupled with Axiocam 503 mono camera. Hoechst-stained heterotrophic bacteria were ...
(g–j) Suspension cell cultures under a microscope for the following species: (g) Alcea kusariensis; (h) Ajuga reptans; (i) Polyscias filicifolia; and (j) Nicotiana tabaccum. Photographs © IPPRAS. Figure 3. Plant families represented in the All-Russian Collection of Plant Cell ...