To elucidate whether interaction between CXCR3 and its ligands is required for the development of SS, we administrated an anti-CXCR3 blocking antibody (CXCR3-173) to the non-obese diabetic (NOD) mice, a well-defined model of SS, during the stage prior to disease onset. Treatment with this...
产品名称:Anti-CXCR3 Antibody 产品型号: 产品展商:其它品牌 产品文档:无相关文档 简单介绍 Anti-CXCR3Antibody Anti-CXCR3 Antibody的详细介绍 Target Function:Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating...
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Sections were boiled with citric buffer (pH 6.0) for 10 min and washed with PBS containing 0.1% Triton-X 100 before standard blocking and antibody incubation procedures. After washing in PBS, Alexa-555 and Alexa-488 fluorescence-conjugated secondary antibodies (Invitrogen; 1:200) were used to ...
CXCR3 receptor blocking was achieved by administration of 63 μg of anti-CXCR3 admixed with 10 μl of virus challenge dose and a second injection of 70 μg of anti-CXCR3 antibody was given at the DTH site 11 h post-challenge. In all cases, IgG controls were used at an equivalent ...
Initial studies focused on CXCR3 requirements for the entry of adoptively transferred effector T cells in competitive (1 h) homing assays in which WT OT-I cells were admixed with Cxcr3 À / À OT-I T cells or with OT-I cells pre-treated with a CXCR3-blocking antibody (Ab). ...
as previously reported25. To avoid false-positive staining due to potential binding of the HCV E2 antigen with CD81 molecules expressed on B cells, cells were treated with an anti-CD81 blocking antibody (JS-81) before HCV E2c tetramer staining. To define the background and baseline of E2c ...
Briefly, the standards and supernatants collected from stimulated Jurkat cells and CD4+ T cells were suspended on a plate pre-coated with each antibody and incubated for 2 h. After washing three times with PBS-T, the detection antibody and streptavidin-HRP solution were added to the plate for...
(5 µm) were deparaffinized, dehydrated, and processed with heat-mediated antigen retrieval using citrate buffer (pH 6.0) followed by a blocking step (Protein block, ab64226, Abcam, Cambridge, UK). Sections were incubated overnight at 4°C with anti-CXCR3 antibody (NB100-56404, Novus ...
Singh UP, Singh S, Singh R, Cong Y, Taub DD, Lillard JW: CXCL10-producing mucosal CD4+ T cells, NK and NKT cells are associated with chronic colitis in IL-10-/- mice, which can be abrogated by anti-CXCL10 antibody inhibition. J Interferon Cytokine Res. 2008, 28: 31-43. 10.1089/...