1.Cre重组酶脑部的表达情况脑部组织免疫荧光染色Cre+（Cx3cr1-iCre[KI/+];Rosa26-LSL-tdTomato[CKI/+]）小鼠在脑部组织中存在大量的tdTomato蛋白红色荧光，其表达与小胶质细胞标记物IBA1的表达的存在共定位，表明在脑部小胶质细胞中存在大量的Cre重组酶表达，而对照组（Cre-）小鼠中检测不到tdTomato红色荧光的...
Cx3cr1-2A-tdTomato-IRES-CreERT2 品系全名 C57BL/6Smoc-Cx3cr1em1(2A-tdTomato-IRES-CreERT2-WPRE-pA)Smoc 目录号 NM-KI-242749 品系状态 活体 导出PDF 基因信息 基因名 Cx3cr1 基因曾用名 - NCBI ID 13051 MGI ID 1333815 Ensembl ID
Temporal genetic labeling (Cx3cr1-CreERT2;R26-tdTomato) showed that CX3CR1+ cells at E6.5 can contribute to cardiomyocytes (CMs) and endothelial cells (ECs) during prenatal development via both de novo differentiation and fusion with pre-existing CMs or ECs, respectively. In the adult heart...
For further validation, we also used a second Cx3cr1 reporter mouse line, in which a tamoxifen-dependent Cre recombinase controlled by the Cx3cr1 locus induces the expression of tdTomato (Cx3cr1creER R26-tdTomato). Again, in the spleen of these mice, we observed Ly6ChighCX3CR1tdTomato...
Fluorescent reporter labeling and promoter-driven Cre-recombinant technologies have facilitated cellular investigations of physiological and pathological processes, including the widespread use of the Cx3cr1CreER-Eyfp/wt mouse strain for studies of micro
图三HexbCreERT2小鼠的小胶质细胞特异性基因重组[7] 综上所述,在生理和病理状态下脑实质小胶质细胞均能特异性表达Hexb基因,使得HexbtdTomato小鼠和HexbCreERT2小鼠可分别用于小胶质细胞的特异性标记和基因重组,为探索小胶质细胞的特征开辟了新途径。
Gpr35-tdTomato mice were crossed with Cx3cr1-GFP mice to generate double reporter mice, and Gpr35flox/flox were crossed with Cx3cr1CreER to obtain Gpr35ΔCX3CR1 mice, in which the tamoxifen-inducible, Cre-mediated recombination will lead to the excision of GPR35 in CX3CR1+ cells. ...
Cx3cr1-2A-tdTomato-IRES-CreERT2 品系全名 C57BL/6Smoc-Cx3cr1em1(2A-tdTomato-IRES-CreERT2-WPRE-pA)Smoc 目录号 NM-KI-242749 品系状态 活体 导出PDF 基因信息 基因名 Cx3cr1 基因曾用名 - NCBI ID 13051 MGI ID 1333815 Ensembl ID
However, based on our study, we consider that the Iba1+tdTomato− cells could be derived from the local microglial cell pool, which does not carry the transgene Cx3cr1CreER. Further- more, another study demonstrated that EYFP− micro- glia escaped Cre-mediated recombination and ...