H: Double-immunofluorescent labeling for the expression of CD1a-positive immature DCs and the intracellular epitope of fractalkine within the cortex of the tonsil. Similar to the pharyngeal epithelium, there is a close association of single CD1a-positive immature DCs (green) with crypt epithelial ...
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There was further no difference in the cell numbers quantified for DCX+ (h) and NeuN+ cells (i) (p > 0.05, two- tailed Student's t-test, cx3cl1+/+ (n = 9) vs. cx3cl1−/− (n = 9)). j 3D-reconstruction of ionized calcium-binding adapter molecule 1 (Iba-1)-labeled ...
When submitted to the tail suspension test 48 and 72 h after LPS, an increased duration of immobility was evident only in CX3CR1-/- mice. This depression-like behavior in CX3CR1-/- mice was associated with a persistent activated microglial phenotype in the hippocampus and prefrontal cortex....
2d). Moreover, macrophages were present in the urine already 4 h after inoculating UPEC into the bladder (Fig. 2e and S4), corroborating the finding of macrophages relocation during acute bacterial infection in the urinary bladder. These findings confirm the SPRING-based results and demonstrate ...
When submitted to the tail suspension test 48 and 72 h after LPS, an increased duration of immobility was evident only in CX3CR1-/- mice. This depression-like behavior in CX3CR1-/- mice was associated with a persistent activated microglial phenotype in the hippocampus and prefrontal cortex....
After washing, DMEM-serum-free medium was added for 24 h, and cells were recovered and centrifuged in order to obtain macrophage-conditioned medium. For phagocytosis studies, cell cultures were performed under standard conditions in DMEM/F12 (Gibco) containing 20% FBS and 2% Ultroser G serum (...
Type I collagen expression was upregulated in C57BL/6 mice after DOCA treatment (b, h). In contrast, the CX3CR1-deficient mice that received DOCA treatment had a reduced expression of type I collagen (b, i). There was no statistical difference in expression levels between sham-operated ...
a novel mechanism by which IFN-γ provokes pregnancy failure Z-Y Li1,2,3, H-H Chao1,2,3, H-Y Liu1, Z-H Song1,2, L-L Li1,2, Y-J Zhang1, Y Yang1 and J-P Peng*,1 Interferon-γ (IFN-γ), a pleiotropic lymphokine, has important regulatory effects on many cell types. ...
The depletion of CX3CR1 + CD11c + cells resulted in an accelerated body weight loss (Figure 5c), increased lethality (Figure 5d), and increased C. rodentium load in MLN, spleen, and liver (Figure 5h). Significant differences between depleted CD11c.DOG, CD11c.DOG×CX3CR1GFP/ + , and ...