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同样,LCI分析表明,当融合蛋白具有相同的定位和直接相互作用时,CwJAZ4/9-cLUC和CwMYC2-nLUC的共转化在烟叶中产生LUC信号(图3d)。 为了证实上述观察结果,进一步进行了体外下拉实验,其中His-CwJAZ4和His-CwJAZ9重组蛋白均被GST‐CwMYC2下拉(图3e)。相反,在对照中没有捕获His-CwJAZ4/9蛋白(没有GST‐CwMYC2作...
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As the master switch of the JA signaling pathway, the CwMYC2-like protein has also been identified and demonstrated to interact with CwJAZ2/3/4/5/7/15/17/20. Further research found that the overexpression of the CwMYC2-like gene increased the accumulation of β-elemene in C. wenyujin ...
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