RT-PCRresults showed that all amplified products showed bright bands at about 250 bp. These results suggest that ra-bies virus infection was the cause of death in mice. The protective effect test results of four different rabiesvaccines on the market without immunoglobulin application showed that ...
A virus neutralization test of 20 samples from both reindeer and caribou showed that ELISA positive samples always neutralized CvHV2 to a greater extent than BoHV1 or elk herpesvirus (ElkHV), indicating that CvHv2 is the most likely virus circulating. PCR of nasal and ocular swabs sampled ...
方法将CVS-11全基因组RNA分成8段进行RT-PCR扩增,分别将产物克隆入pGEM-TEasy载体中,测定全序列;用DNAStar软件包中的相应软件对基因全序列进行分析,并与5株生产用狂犬病病毒(CTN-1、aG、FluryLEP、PM和PV)进行基因比对分析和主要抗原位点比较。结果CVS-11株基因组全长11927bp,其结构基因排列与已知其他狂犬病病毒...
Methods Animprovedrapidfluorescencefocusinhibition test(RFFIT)fordetectionofneutralizingantibodyagainstrabiesvirus(RVNA)wasestablishedbasedon theCVS11pseudovirusexpressingaluciferasereportergene. Fortysixhumanserumsampleswereanalyzed withtheimprovedRFFITandtheresultswerecomparedwiththosebyusingstandardRFFIT. Moreover,the ...
方法将CVS-11全基因组RNA分成8段进行RT-PCR扩增,分别将产物克隆入pGEM-TEasy载体中,测定全序列;用DNAStar软件包中的相应软件对基因全序列进行分析,并与5株生产用狂犬病病毒(CTN-1、aG、FluryLEP、PM和PV)进行基因比对分析和主要抗原位点比较。结果CVS-11株基因组全长11927bp,其结构基因排列与已知其他狂犬病病毒...