51, In summary, we have described a novel attenuated strain of CVB3 called Mt 10 virus that serves as a vaccine candidate in the prevention of both myocarditis and pancreatitis in the mouse model of CVB3 infection. The Mt 10 virus induces IgG nABs accompanied with the generation of IFN-γ...
进一步如上文所述的方法,其中所述cvb3病毒选自cvb3nancy、31-1-93、h3和pd的组,所述cvb3nancy、31-1-93、h3和pd全部特征在于在选自包括病毒衣壳蛋白1(vp1)中的氨基酸残基k78、a80、a91和i92的组的氨基酸序列中的至少一种或几种改变。进一步如上文所述的方法,其中所述cvb3病毒是cvb3pd-0或其经修饰的形式。
An infectious cDNA of a highly myocarditic coxsackievirus B3 (CVB3m; Nancy strain) was cloned. Sequence data revealed 43 extra non-viral nucleotides upstream of the initial 5' sequence. However, the authentic 5' end sequence was maintained during replication of viral RNA transfected into HeLa ...
Coxsackievirus B3 (CVB3) Nancy strain was obtained from Shanghai Jiao Tong University School of Medicine, propagated in H9C2 cells and stored at −80 °C in our laboratory. The titer of virus was examined prior to each experiment. Cells in CVB3 groups were infected at 100 TCID50 with CV...
Oral vaccine is highly desired for infectious disease which is caused by pathogens infection through the mucosal surface. The design of suitable vaccine delivery system is ongoing for the antigen protection from the harsh gastric environment and target t
1OOTCID50 of coxsackie virus B3 (CVB3) (Nancy strain )was added as the infectedgroup. The dynamics of taurine transport across membrane of normal heart cells and changes after infectionwere examined by using radioactive isotope tracing techniques. The results demonstrated that: (1)two uptake ...
I00TCID50, of eoxsaekie virus B3 (CVB3)(Nancy strain)was added as the infected group. The dynamics of taurine transport across membrane of normal heart ceils and changes after infection were examined hy using radioactive isotope tracing techniques, The results demonstrated that:(1)two uptake ...
To examine the role of CD4 + T cells and especially the role of the T helper 1 cell response for the outcome and pathogenesis of CVB3-induced myocarditis in more detail, 2 different mouse strains with identical genetical background (H-2 b ) were infected with CVB3-M眉/J (Nancy strain)...
CVB3 Nancy induced higher viral titers than did the mutant strain. There was no variation of the neutralizing activity of serum taken from mice immunized with CVB3 Sabin3-like and challenged with CVB4 E2, compared to non-immunized mice. Despite the fact that CVB3 and CVB4 are closely related...
CVB3 (Nancy strain) was kindly provided by Professor Yingzhen Yang (Key Laboratory of Viral Heart Diseases, Zhongshan Hospital, Shanghai Medical College of Fudan University, Shanghai, China), and was serially passaged in HeLa cells. Murine peritoneal macrophages were collected from C57BL/6 mice ...