• Protein purity is determined by SDS-PAGE, and quantity is determined by Bradford assay. • Protein is delivered in freeze powder whenever possible (in neutral buffer before lyophilization: 1x PBS, pH 7.4). If protein is delivered in solution form, the default buffer is: 1 x PBS, 50...
purification and characterization. Our purification strategy aims to achieve a homogeneous active protein preparation in two to three purification steps. This goal is reached by a thorough selection and optimization of the capture step, incorporation of a gel filtration step to remove aggregates, degrad...
Protein Purification Protein Characterization Functional Assays & Assay Development ELISA Development Protein Labeling & Conjugation Endotoxin Removal & Testing Antibody Development & Production Start a Project Get a Quote How to Start a Project Forms & Agreements ...
Purification/buffer exchangeIndividual fractionsDOL optimizationCustom final packaging/formulation Optional add-on services include BSA removal Ascites fluid purification Unique buffer formulations Individual fractions with a fraction letter 6 MRs (wide) 3 MRs (narrow) Lyophilization Non-...
Analytics plays a key role in protein production in order to get functional proteins with high purity. Though the challenge is with the latter, many hard-to-express antigens require very cutomized approach from purification to storage buffer formulation. For each step we have highly qualified pers...
Need larger or smaller aliquots than our standard size? Require reagent at a higher concentration or in an alternative buffer? We can do it, delivering what your project needs to be successful. Expert technical support Customizable to suit your needs ...
Purification Method: Affinity purification and molecular sieve chromatography Method Used to Determine Concentration: OD280 or Bradford Assay Buffer: PBS Concentration: ~1mg/mL Amount: To produce a nanobody to your protein, we require: 1-1.5mg of purified protein in PBS or HBS Sample pur...
Biology macromolecules can be collected in any SEC buffer with the presents of cofactors, detergents, essential metal ions, denaturants, etc. With years of experience on macromolecule purification, Creative Biostructure provides custom size-exclusion chromatography service with high quality for various...
Send 50%micro;l of each protein at concentration 0.1-1mg/ml. In addition, please include a 50µl aliquot of your sample buffer. Ship on dry ice to: Custom Array Service 688 East Main Street Branford, CT 06405 8) Proteins derived from Invitrogen’s...
Antibody buffer exchanged to remove interfering materials ● ● Add-on size exclusion chromatography to remove aggregates (optional) ● Pre-conjugation analytical reagent characterization ● Conjugation according to optimized procedures under MSD’s Quality Management System ● ● Post-conjugation purifica...