A stock solution (5 mM) of capsaicin was made in ethanol and diluted in the external solution. Capsaicin was applied to DRG neurons using the fast perfusion SF77-B system (Warner Instruments, Hamden, CT, USA). To record TTX-resistant sodium currents, the external solution had the ...
ATP—activatedcurrentinnociceptiveneurons. Keywords:P2X;phenotype;genotype;tooth—pulp;trigeminalganglion;retrogradefluores- cence—tracing;immunohistochemistry;nociceptor ExtracellularATPhasbeenfoundtobeapotentially importantexcitatorymediatorinthenervoussystem,and ...
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[21]. Cells were transferred to a temperature controlled recording chamber in which they were continuously perfused with the bath solution. Temperature was maintained at 37 ± 0.5 °C by a temperature control system (TC-324B, Warner, Hamden, CT, USA). Whole-cell patch-clamp recordings were ...
2.3. Electrophysiological Recordings in CGN Neurons Macroscopic currents obtained from CGNs were studied using the whole-cell patch-clamp configuration with a PC-501A amplifier (Warner Instruments, Hamden, CT, USA), as described previously [27]. The pCLAMP10 with an acquisition card (DigiData 1440...
2.3. Electrophysiological Recordings in CGN Neurons Macroscopic currents obtained from CGNs were studied using the whole-cell patch-clamp configuration with a PC-501A amplifier (Warner Instruments, Hamden, CT, USA), as described previously [27]. The pCLAMP10 with an acquisition card (DigiData 1440...
The trans compartment was connected to the head-stage input of a Warner BC-535D amplifier (Warner Instruments, Inc., Hamden, CT, USA), and the cis compartment was held at the ground level. Electrical signals were filtered through the Warner BC-535D low-pass Bessel filter at 1 kHz and ...
The trans compartment was connected to the head-stage input of a Warner BC-535D amplifier (Warner Instruments, Inc., Hamden, CT, USA), and the cis compartment was held at the ground level. Electrical signals were filtered through the Warner BC-535D low-pass Bessel filter at 1 kHz and ...