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The SYBR Green PCR Master Mix kit was purchased from Applied Biosystems (Foster City, CA, USA). Primary neuronal culture of the dorsal root ganglion Animals were handled according to protocols approved by the Animal Care and Use Committee of Chang Gung University. Three-week-old Sprague–Dawley...
0.5 μM of forward primer labelled either with 6: FAM, HEX, NED or PET fluorophore, 0.5μM of non-labelled reverse primer, 150 μM of each dNTP (Boehringer, Manheim, Germany), 2.5 mM MgCl2, 1X buffer ampliTaq and 0.8 units AmpliTaq polymerase (PE/Applied Biosystems, Foster City, CA...
Ding L, Mao RF, Ma SR, Guo XT, Zhu LY (2020a) High temperature depended on the ageing mechanism of microplastics under different environmental conditions and its effect on the distribution of organic pollutants. Water Res 174:115634. https://doi.org/10.1016/j.watres.2020a.115634 Article CA...
All experiments were performed at room temperature (21–23°C). Glass suction pipettes were fabricated from borosilicate capillary glass using a Flaming-Brown type micropipette puller (model P-87; Sutter Instrument Co., Novato, CA). When filled with pipette solution, electrodes had resistances of ...
efficiency in energy production (increasing energy gain by up to 16%) compared with using conventional solar roofs, as this combination eliminates pollutants in the air while maintaining the panel surface at an optimum temperature range via cooling (Barcelona City Council2015; Cavadini and Cook2021)...
After digestion the slices were washed six times with external solution and incubated in the same solution saturated with pure O2 at room temperature. CA1 regions were dissected out and trans- ferred into centrifuge tubes. Hippocampal neurons were dis- persed by gentle pipetting using fine glass...
Precipitation and humidity could provide the necessary water environment and aero- sols for virus survival, and protect the virus from harm- ful factors such as temperature, salinity, and pH [35]. The intensity of UV exposure time also affected the incidence of HFMD [36]. Also, the terrain ...
Projections of future GMSL change are generally based on either a bottom-up assessment of the different contributory processes or on top-down semi-empirical methods [e.g., 104], which are based on the past relationship between temperature and rates of GMSL change; top-down reasoning by analogy...
The two solutions were then added together and incubated at room temperature for 5 min before being added to the HEK293 cells. The siRNA was added such that the final concentrations in the 24 well plate was 3, 10 or 30 μM. Evaluation of the suppression efficiencies of the miRNA ...