This unit describes the ChIP protocol for Saccharomyces cerevisiae; however, it is also applicable to other organisms.doi:10.1002/0471143030.cb1707s23Oscar AparicioJoseph V GeisbergKevin StruhlCurrent protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]...
In addition, ChIP can be combined with microarray technology to identify the location of specific proteins on a genome-wide basis. This unit describes the ChIP protocol for Saccharomyces cerevisiae; however, it is also applicable to other organisms. 展开 ...
The Basic Protocol describes subculturing of a monolayer culture grown in petri plates or flasks; the Alternate Protocol 1 describes passaging of suspension cultures. Support Protocols describe freezing of monolayer cells, thawing and recovery of cells, counting cells using a hemacytometer, and ...
PROTOCOL Open access Analyzing Pathophysiology and Immune Cells and Their Cytokines and Mediators in Precision‐Cut Slices of the Murine Lung Michaela Tatcheff,Christine Carvalho,Jonas Willar,Elvedina Nendel,Susanne Krammer,Mircea T. Chiriac,Shuting Zhou,Carol I. Geppert,Susetta Finotto ...
Basic Protocol 1: STARR-seq plasmid library cloning Basic Protocol 2: Mammalian STARR-seq screening protocol Alternate Protocol: UMI-STARR-seq screening protocol—unique molecular identifier integration Support Protocol: Transfection of human cells using the MaxCyte STX scalable transfection systemINTRODUCTION...
A support protocol describes the preparation of multiply deficient medium (MDM) for use in making appropriate deficient media.doi:10.1002/0471142735.im0813s09Sandra DíazA. VarkiWileyCurrent Protocols in Immunology... S Díaz,A Varki - 《Current Protocols in Immunology》 被引量: 0发表: 1994年 ...
Current Protocols in CytometryMcCoy JP Jr. Handling, storage, and preparation of human blood cells. Curr Protoc Cytometry 1998; vol. 5:5.1.1-5.1.13... PK Dagur,JP Mccoy - 《Current Protocols in Cytometry》 被引量: 16发表: 2015年 Consensus protocol for the flow cytometric immunophenotyping ...
Many units in the manual contain groups of protocols, each presented with a series of steps. ABasic protocol is presented first in each unit and is generally the recommended or most universally applicable approach. Additional basic protocols may be included where ...
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