The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.FEBS LettersJeong, Jong-JinFushinobu, ShinyaIto, SoheiJeon, Beong-SamShoun, HirofumiWakagi, Takayoshi
The relative position and number of histidines in the structure point to a metal ion containing protein. In addition, the exact metal ion remains to be determined but our study points to manganese or zinc. The Cupin superfamily is very large with diverse functions. Many of these functions ...
Straganza GD, Eggera S, Aquinoc G, D'Auriac S, Nidetzkya B: Explor- ing the cupin-type metal-coordinating signature of acetylac- etone dioxygenase Dke1 with site-directed mutagenesis: Catalytic reaction profile and Fe2+ binding stability of Glu-69 Gln mutant. Journal of Molecular Catalysis...
Fig. 1: Selected natural products containing a nitrogen-nitrogen bond and enzymatic N-N bond formation by a family of di-domain proteins. It has been noticed that a collection of three genes, encoding a lysine/ornithineN-hydroxylase, a cupin protein, and a methionyl-tRNA synthase (MetRS) hom...