Here we investigated these factors in a human cell model harboring 800 CTG•CAG repeats by individually knocking down various DNA repair proteins using short interfering RNA. Knockdown of MSH2 and MSH3, which form the MutSβ heterodimer and function in mismatch repair, suppressed large repeat ...
CTGCAG中有2个CG对,1个TA对 所以概率是 P(CG)P(CG)P(AT)=0.4*0.4*0.6=0.096
CTGCAG -- C TCGTC(3)将PCR产物片段与线性质粒载体混合后,在重组酶作用下可形成环化质粒,直接用于转化细菌。 这一过程与传统重组质粒构建过程相比,无需使用的酶主要有(4)转化后的大肠杆菌需采用含有抗生素的培养基筛选,下列叙述错误的有 A.稀释涂布平板需控制每个平板30~300个菌落 B.抗性平板上未长出菌落的...
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如需连接上两末端Pst I末端还需用S1核酸酶降解末端才能进行平端连接。EcoRI的末端为:5"-GAATTC-3"3"-CTTAAG-5"PstI的末端为:5"-CTGCAG-3"3"-GACGTC-5"经DNA聚合酶作用后,EcoRI末端能用DNA聚合酶填平,但PstI不行。如需连接上两末端,PstI末端还需用S1核酸酶降解末端,才能进行平端连接。
The properties of duplex CTG·CAG and CGG·CCG, which are involved in the etiology of several hereditary neurodegenerative diseases, were investigated by a variety of methods, including circularization kinetics, apparent helical repeat determination, and polyacrylamide gel electrophoresis. The bending ...
2023年视频,美军JSOC-CAG和库尔德CTG部队在伊拉克北部联合行动清剿“伊斯兰国”武装。 http://t.cn/A6Cnxxpb
CAG·CTG repeat instability in cultured human astrocytes Cells of the central nervous system (CNS) are prone to the devastating consequences of trinucleotide repeat (TNR) expansion. Some CNS cells, including astr... BT Farrell,RS Lahue - 《Nucleic Acids Research》 被引量: 38发表: 2006年 The...
Expansion of (CTG)n•(CAG)n trinucleotide repeat (TNR) microsatellite sequences is the cause of more than a dozen human neurodegenerative diseases. (CTG)n and (CAG)n repeats form imperfectly base paired hairpins that tend to expand in vivo in a length-
Expansion of (CTG)•(CAG) repeats, the cause of 14 or more diseases, is presumed to arise through escaped repair of slipped DNAs. We report the fidelity of slipped-DNA repair using human cell extracts and DNAs with slip-outs of (CAG)20 or (CTG)20. Three outcomes occurred: correct ...