CRISPR/Cas and other genome editing technologies have some problems, such as large molecular weight of targeted nuclease, high off-target rate, and limited by PAM site. To solve these problems and construct new mini genome editing system based on Csy4 and MCP, AlphaFold2 was used to predict ...
Here, we used these two viral proteins to shuttle CRISPR-Csy4 nuclease to RV viroplasms and to obtain the nuclease-mediated site-specific genome editing of a dsRNA virus. Using different recombinant RVs (rRVs) carrying the Csy4 target sequence in diverse positions and viral segments, we ...
site_5_destroyed entrez_gene genbank_ids KJ796485 mutation nameCsy4/Cas6 shRNA_sequence size558 species 9606 Homo sapiens tags locationN terminal on insert tag6xHIS resistance markers : 1726 tags : Unknown more info or order: Addgene product webpage ...
The active site catalytic dyad lacks a general acid to protonate the leaving group and positively charged residues to stabilize the transition state, explaining why the observed catalytic rate constant is 104-fold slower than that of RNase A. We show that this RNA cleavage step is essential for...
(sgRNA).1 The pegRNA contains a primer binding site (PBS) and a reverse transcription (RT) template for introducing new genetic informa-tion1 (Fig.1a;Supplementary information,Fig.S1a).We noted that the PBS,which is generally 10-16 nt at the 3'end of pegRNA,is complementary to part of...
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The bacterial defense system CRISPR (clustered regularly interspaced short palindromic repeats) has been explored as a powerful tool to edit genomic elements. In this study, we test the potential of CRISPR Csy4 RNA endoribonuclease for targeting HIV-1. We fused human codon-optimized Csy4 endoribon...
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